Terbuthylazine (TBA), atrazine (ATZ) (both Pestanal, purity 99.1%) and urea (≥ 95%) were purchased from Sigma-Aldrich (Seelze, Germany), (NH4)2SO4 from Panreac (Barcelona, Spain) and NaNO3 from Merck (Darmstadt, Germany). The formulated herbicide Terbutilazina-Sapec (concentrate suspension of 0.5 kg active substance L-1; recommended field dose (RD) for weed control in corn plantations of 1.5 L ha-1) was purchased from Sapec—Agro (Setúbal, Portugal).
Nh4 2so4
Manufactured by ITW Reagents
Sourced in Germany
Ammonium sulfate, chemical formula (NH4)2SO4, is a crystalline solid inorganic compound. It is a common laboratory reagent used in various applications, such as precipitation and crystallization experiments.
Automatically generated - may contain errors
Lab products found in correlation
L methionine, by Merck Group (2 mentions)
L histidine, by Merck Group (2 mentions)
L leucine, by Merck Group (2 mentions)
L uracil, by Merck Group (2 mentions)
Urea, by Merck Group (1 mentions)
Terbuthylazine, by Merck Group (1 mentions)
Nano3, by Merck Group (1 mentions)
Yeast extract, by BD (1 mentions)
Bacto peptone, by BD (1 mentions)
K2hpo4, by ITW Reagents (1 mentions)
Na2hpo4, by ITW Reagents (1 mentions)
C6h8o7, by ITW Reagents (1 mentions)
C2h4o2, by ITW Reagents (1 mentions)
Phytic acid, by Merck Group (1 mentions)
L lactic acid, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Succinic acid, by Merck Group (1 mentions)
Butyric acid, by Merck Group (1 mentions)
Benzoic acid, by Merck Group (1 mentions)
Fumaric acid, by Merck Group (1 mentions)
6 protocols using nh4 2so4
1
Herbicide Degradation Experiment Protocol
2
Cultivation of Saccharomyces and Candida Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
S. cerevisiae BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0) and the derived deletion mutants pdr18Δ and snq2Δ were obtained from EUROSCARF collection. C. glabrata BPY55 (clinical isolate) and the derived deletion mutant snq2Δ built using the SAT1 flipper system were kindly provided by Professor Dominique Sanglard, Institut de Microbiologie, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland.
Cultivation of S. cerevisiae strains was performed in MM4 medium, containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulfate (Difco, Detroit, MI, United States), 20 g/L glucose (Merck, Darmstadt, Germany), 2.65 g/L (NH4)2SO4 (Panreac AppliChem, CT, United States), 20 mg/LL -methionine, 20 mg/L L -histidine (both from Merck, Darmstadt, Germany), 60 mg/L L -leucine and 20 mg/L L -uracil (both from Sigma, St. Louis, MO, United States). C. glabrata strains were cultivated in MM medium, with the composition of MM4 medium, without supplementation with amino acids and uracil. YPD medium contained 20 g/L glucose, 20 g/L BactoTM Peptone and yeast extract (both from BD Biosciences, Franklin Lakes, NJ, United States). Solid media were prepared by the addition of 20 g/L agar (Iberagar, Barreiro, Portugal) to the different liquid media. Media pH were adjusted to 4.5 with HCl. Growth in liquid media was performed at 30°C with orbital agitation (250 rpm).
Cultivation of S. cerevisiae strains was performed in MM4 medium, containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulfate (Difco, Detroit, MI, United States), 20 g/L glucose (Merck, Darmstadt, Germany), 2.65 g/L (NH4)2SO4 (Panreac AppliChem, CT, United States), 20 mg/L
3
Yeast Strain Cultivation and Complementation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Saccharomyces cerevisiae parental strain BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0) and the derived deletion mutant pdr18Δ were obtained from the EUROSCARF collection (http://web.uni-frankfurt.de/fb15/mikro/euroscarf), as well as plasmid pYCG_PDR18, expressing the PDR18 gene from its natural promoter, and the corresponding cloning vector, pRS416, used for phenotypic complementation assays.
Yeast cells were cultivated at 30 °C with orbital agitation (250 rpm) in liquid minimal growth medium supplemented with the amino acids and the nucleotide to support growth of the auxotrophic strains (MM4). MM4 contained 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Difco, Michigan, USA), 20 g/L glucose (Merck, Darmstadt, Germany), 2.65 g/L (NH4)2SO4 (Panreac AppliChem, Connecticut, USA), 20 mg/L L-methionine, 20 mg/L L-histidine (both from Merck, Darmstadt, Germany), 60 mg/L L-leucine and 20 mg/L L-uracil (both from Sigma, Missouri, USA), adjusted to pH 4.0 with HCl. Solid medium was prepared by the addition of 20 g/L agar (Iberagar, Barreiro, Portugal) and the pH was set to 4.5 with HCl. Cells harboring the cloning vector pRS416 or derived plasmids were grown in the same medium lacking uracil supplementation (MM4-U medium) to maintain selective pressure.
Yeast cells were cultivated at 30 °C with orbital agitation (250 rpm) in liquid minimal growth medium supplemented with the amino acids and the nucleotide to support growth of the auxotrophic strains (MM4). MM4 contained 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Difco, Michigan, USA), 20 g/L glucose (Merck, Darmstadt, Germany), 2.65 g/L (NH4)2SO4 (Panreac AppliChem, Connecticut, USA), 20 mg/L L-methionine, 20 mg/L L-histidine (both from Merck, Darmstadt, Germany), 60 mg/L L-leucine and 20 mg/L L-uracil (both from Sigma, Missouri, USA), adjusted to pH 4.0 with HCl. Solid medium was prepared by the addition of 20 g/L agar (Iberagar, Barreiro, Portugal) and the pH was set to 4.5 with HCl. Cells harboring the cloning vector pRS416 or derived plasmids were grown in the same medium lacking uracil supplementation (MM4-U medium) to maintain selective pressure.
4
Bacterial Cellulose Membrane Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cellulose membrane was synthesized by a bacterial cellulose producer, the Gluconacetobacter hansenii GH-1/2008 (VKPM B-10547) strain [30 ]. The culture of bacteria strain GH-1/2008 was grown at 28 ± 2 °C for 5–7 days in the following medium: 30 g sucrose, 2.7 g disodium hydrogen phosphate (Na2HPO4 (PanReac AppliChem, Darmstadt, Germany)), 2 g dipotassium hydrogen phosphate (K2HPO4 (PanReac AppliChem, Darmstadt, Germany)), 3 g ammonium sulfate ((NH4)2SO4 (PanReac AppliChem, Darmstadt, Germany)), 1.15 g citric acid (C6H8O7 (PanReac AppliChem, Darmstadt, Germany)), 1% ethyl alcohol and 5 g yeast extract per 1 L of purified water. The derived 500 μm thick membranes were washed in running water, then in a 0.5 N solution of sodium hydroxide (NaOH (PanReac AppliChem, Darmstadt, Germany)) for 20 min at +40 °C, and neutralized with a 0.5 N acetic acid solution (C2H4O2 (PanReac AppliChem, Darmstadt, Germany)). The membranes were repeatedly washed with distilled water. The membranes were stored in distilled water until usage.
5
Fungal culture media preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ultrapure water (conductivity 18.2 mΩ) was used in all experiments. For fungal cultures, malt extract was purchased from VWR, EDTA-Na2 and glucose were from Sigma, (NH4)2SO4 and CaCl2 were from Panreac, H3BO3, MnCl2, FeSO4, CuSO4, CoCl2 Na2MoO4, MgSO4, KH2PO4, and ZnSO4 were purchased from Prolabo and Bacto agar was purchased from Fischer.
For analytic methods, CD3OH, HPLC grade organic acid standards (acetic, adipic acid, L-ascorbic acid, benzoic acid, butyric acid, citric acid, isobutyric acid, formic acid, fumaric acid, L-(+)-lactic acid, DL-isocitric acid trisodium salt hydrate, maleic acid, malonic acid, D-(+)-malic acid, oxalic acid, phytic acid, propionic acid, (−)quinic, succinic acid, shikimic acid,D-(−)-tartaric acid), methylchloroformate (MCF), and glucose were purchased from Sigma, HPLC grade ethanol was purchased from Fluka. Dichloromethane was from Carlo Erba Reagents.
For analytic methods, CD3OH, HPLC grade organic acid standards (acetic, adipic acid, L-ascorbic acid, benzoic acid, butyric acid, citric acid, isobutyric acid, formic acid, fumaric acid, L-(+)-lactic acid, DL-isocitric acid trisodium salt hydrate, maleic acid, malonic acid, D-(+)-malic acid, oxalic acid, phytic acid, propionic acid, (−)quinic, succinic acid, shikimic acid,D-(−)-tartaric acid), methylchloroformate (MCF), and glucose were purchased from Sigma, HPLC grade ethanol was purchased from Fluka. Dichloromethane was from Carlo Erba Reagents.
6
Yeast Strain Cultivation and Acetic Acid Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The S. cerevisiae parental strain BY4741 (MATa, his3Δ1, leu2Δ10, met15Δ0, ura3Δ0), obtained from the EUROSCARF collection, was used in this study. The strain was maintained at − 80 °C in YPD media supplemented with 30% (v/v) glycerol. Cells were cultivated at 30 °C, with orbital agitation (250 rpm), in minimal growth medium supplemented with amino acids and uracil (MM4). MM4 contains 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Difco, Michigan, USA), 20 g/L glucose (Merck, Darmstadt, Germany), 2.65 g/L (NH4)2SO4 (Panreac AppliChem, Connecticut, USA), 20 mg/L methionine, 20 mg/L histidine, 60 mg/L leucine and 20 mg/L uracil (all from Sigma, Missouri, USA). The medium pH was adjusted to 4.0 with HCl. For the majority of the experiments performed under acetic acid stress, MM4 medium was supplemented with 60 mM acetic acid (Fluka, Waltham, USA) using a solution of 5 M acetic acid, set to pH 4.0 with NaOH. For the majority of the growth experiments, MM4 was inoculated to an initial optical density at 600 nm (OD600nm) of 0.1 ± 0.05 mid-exponential cells harvested by filtration (Whatman, Maidstone, UK) from cultivation in fresh MM4 medium (pH 4.0) without acetic acid supplementation. Growth was followed by measuring culture OD600nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!