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Control rat igg2b clone ltf 2

Manufactured by BioXCell

Control rat IgG2b (clone LTF-2) is a laboratory reagent used as an isotype control for flow cytometry and other immunoassays. It is a purified rat monoclonal antibody of the IgG2b subclass, which can be used to establish background staining levels.

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2 protocols using control rat igg2b clone ltf 2

1

TCM Compounds and Immune Modulators

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Compounds purified from various TCMs, including CT, were obtained from the National Institutes for Food and Drug Control (Beijing, China). CT was dissolved at a stock concentration of 15 ~ 20 mg/ml in DMSO and diluted into physiologic solutions or medium for experiments. LPS (E. coli O55:B5) was from Sigma. Anti-PD-L1 (clone 10F.9G2) and control rat IgG2b (clone LTF-2) were purchased from BioXCell (West Labanon, NH).
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2

PD-L1 Blockade and Lymphocyte Depletion

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For PD-L1 blockade, anti-PD-L1 Ab (clone 10F.9G2, BioXCell) or isotype control rat IgG2b (clone LTF-2, BioXCell) were given intraperitoneally every three days from the day RT performed at a dose of 200 μg/mouse for three times (37 ). For in vivo depletion of lymphocytes, 200 μg of anti-CD4 (clone GK1.5, BioXCell), anti-CD8β (clone Lyt 3.2, BioXCell), or control rat IgG2b (clone LTF-2, BioXCell) were injected intraperitoneally every three days from the day when RT was given for three times (37 ). Depletion of each subset was confirmed on day 2 (data not shown).
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