Cells were lysed with RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with phosphatase and protease inhibitor cocktails (Roche Diagnostics, Basel, Switzerland). SDS‐PAGE (10%) and semi‐dry transfer were carried out using standard procedures. Membranes were incubated at 4°C overnight with primary monoclonal antibodies including mouse anti‐ASPH (FB‐50),17 rabbit anti‐β actin (D6A8; Cell Signaling Technology), rabbit anti‐ERK1/2 (137F5; Cell Signaling Technology), rabbit anti‐P‐ERK1/2 (Thr202/Tyr204; D13.14.4E; Cell Signaling Technology), rabbit anti‐Akt (C67E7; Cell Signaling Technology), and rabbit anti‐P‐Akt (Ser473; D9E; Cell Signaling Technology). All primary antibodies were raised against human proteins. Membranes were incubated with HRP‐conjugated anti‐mouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) or anti‐rabbit IgG antibody (Cell Signaling Technology), followed by chemiluminescence detection. Acquired images were processed using ImageJ (1.48v; National Institutes of Health, Bethesda, MD, USA), and ACTB/β actin (D6A8; Cell Signaling Technology) was used as a loading control.
Rabbit anti akt c67e7
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti‐Akt (C67E7) is a primary antibody that specifically recognizes Akt, a serine/threonine protein kinase involved in various cellular processes. This antibody is produced in rabbit and purified by protein A and peptide affinity chromatography.
Automatically generated - may contain errors
Lab products found in correlation
Protease and phosphatase inhibitor cocktail, by Roche (1 mentions)
Anti rabbit igg antibody, by Cell Signaling Technology (1 mentions)
Ripa lysis and extraction buffer, by Thermo Fisher Scientific (1 mentions)
Rabbit anti erk1 2 137f5, by Cell Signaling Technology (1 mentions)
Rabbit anti p akt ser473 d9e, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti mouse igg antibody, by Jackson ImmunoResearch (1 mentions)
Sp600125, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
3 methyladenine, by Merck Group (1 mentions)
Mouse anti gapdh, by Beyotime (1 mentions)
Kb r7943, by Merck Group (1 mentions)
Wortmannin, by Beyotime (1 mentions)
Rabbit anti ki67 ab15580, by Abcam (1 mentions)
2 protocols using rabbit anti akt c67e7
1
Immunoblotting of ASPH and Signaling Proteins
2
Evaluation of Cellular Stress Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rabbit anti-SQSTM1/P62 (D5E2), rabbit anti-LC3A/B (D3U4C), rabbit anti-mTOR (7C10), rabbit anti-p-mTOR (Ser2448), rabbit anti-p-SAPK/JNK (Thr183/Thr185), rabbit anti-SAPK/JNK (56G8), rabbit anti-cyclinD1 (96G2), rabbit anti-p-ERK (Thr202/Thr204), rabbit anti-ERK (137F5), rabbit anti-p-P38 (Thr180/Tyr12), rabbit anti-P38 (D13E1), rabbit anti-p-AKT (Ser473, D9E), and rabbit anti-AKT (C67E7) were from Cell Signaling (Danvers, MA, USA). Rabbit anti-GRP78 (ab21685), rabbit anti-IRE1 (ab37073), rabbit anti-NCX1 (ab151608), and rabbit anti-Ki67 (ab15580) were from Abcam (Cambridge, MA, USA). Mouse anti-GAPDH was from Beyotime Biotechnology (Nanjing, China). NCX1 inhibitor KB-R7943 (Sigma, St Louis, MO, USA, K4144-25MG) was dissolved in DMSO at 10 mM. Autophagy inhibitor 3-methyladenine (Sigma, M9281-100MG) was dissolved in medical injectable water at 50 mM. Chloroquine (Sigma, C6628-25G) was dissolved in medical injectable water at 10 mM. Wortmannin (Beyotime Biotechnology, Nanjing, China) and SP600125 (Sigma, S5567-10MG) were dissolved in DMSO at 10 mM.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!