Plk1 clone 35 206

Manufactured by Merck Group

Sourced in United Kingdom

The PLK1 clone 35-206 is a lab equipment product manufactured by Merck Group. It is a cloned genetic sequence related to the Polo-like kinase 1 (PLK1) protein. The core function of this product is to provide a research tool for studying the PLK1 protein, which plays a crucial role in cell division and cell cycle regulation. The detailed specifications and intended applications of this product are not available.

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Lab products found in correlation

Pericentrin, by Abcam (2 mentions) Nocodazole, by Merck Group (1 mentions) Vinculin, by Merck Group (1 mentions) α tubulin dm1a, by Merck Group (1 mentions) γ tubulin gtu 88, by Merck Group (1 mentions) Nedd1, by Abcam (1 mentions) Acetyl tubulin 6 11b 1, by Santa Cruz Biotechnology (1 mentions) Bubr1, by Cell Signaling Technology (1 mentions) Mg132, by Merck Group (1 mentions) Flag hrp, by Merck Group (1 mentions) Alpha tubulin clone dm1a, by Merck Group (1 mentions) Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Amersham ecl, by GE Healthcare (1 mentions) Gamma tubulin, by Abcam (1 mentions) Flag m2, by Merck Group (1 mentions)

2 protocols using plk1 clone 35 206

Antibody Profiling for Microtubule Proteins

Cited 1 time

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Rabbit antibodies against tubulin C102 were a gift of Dr. M. Andreu and HEC1 antibodies were a gift of Dr. PT. Stuckenberg (University of Virginia, Charlottesville, VA, USA). Polyclonal antibodies against TPX2 and PolyE-Tubulin were home-made [32 (link),33 (link)]. All other antibodies are commercially available: Acetyl-tubulin 6-11B-1 (Santa Cruz, Santa Cruz, CA, USA), α-tubulin DM1A (Sigma, Burlington, MA, USA), Vinculin (Sigma), γ-tubulin GTU88 (Sigma), Kinesin 5 (Abcam, Cambridge, UK), pericentrin (Abcam), NEDD1 (Abcam), PRC1 (Santa Cruz), HURP (Proteintech, Chicago, IL, USA), CREST (Europa Bioproducts, Cambridge UK), PLK1 clone 35-206 (Millipore, Burlington, MA, USA), KIF2A (Novus, Littleton, CO, USA), NuMA (Abcam), BUBR1 (Cell Signaling, Danvers, MA, USA), MCAK (Millipore), MYPT1 (Proteintech).
Most chemicals, including Nocodazole, STLC, and MG132, were obtained from Sigma. BI2356 was purchased from MedChemExpress.

Rasamizafy S.F., Delsert C., Rabeharivelo G., Cau J., Morin N, & van Dijk J. (2021). Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis. Cells, 10(8), 1859.

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Comprehensive Immunoblotting Reagents Catalog

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Alpha-tubulin, clone DM1A (Sigma Aldrich, T9026, mouse monoclonal; RRID: AB_477593); Alpha-tubulin-FITC, clone DM1A (Sigma Aldrich, F2168, mouse monoclonal; RRID: AB_477593); Flag M2 (Sigma Aldrich, F3165, mouse monoclonal, RRID: AB_259529); Flag-HRP (Sigma Aldrich, A8592, mouse monoclonal RRID: AB_439702); Gamma-tubulin (Abcam, 11317, rabbit polyclonal; RRID: AB_297921); GAPDH-HRP (Novus, NB110-40405, mouse monoclonal; RRID: AB_669249); Gravin, clone JP74 (Sigma Aldrich, G3795, mouse monoclonal); Gravin, clone R3698 (in-house (34) , rabbit polyclonal); Nedd1, clone H-3 (Santa Cruz Biotechnology, sc-398733, mouse monoclonal); Pericentrin (Abcam, ab4448, rabbit polyclonal; RRID:AB_304461); Phospho-Plk1 T210 (Biolegend, 628901, rabbit polycolonal; RRID: AB_439786); Phospho-Plk1 T210 clone 2A3 (Abcam, ab39068, mouse monoclonal, RRID: AB_10861033); Plk1, clone 35-206 (Millipore, 05-844, mouse monoclonal; RRID: AB_11213632); Plk1, clone F-8 (Santa Cruz Biotechnology, sc-17783, mouse monoclonal; RRID: AB_628157); Ssecks (BD Transduction Labs, S63820, mouse monoclonal). Donkey anti-Mouse IgG, Alexa Fluor 488 (Invitrogen, A-21202); Donkey anti-Rabbit IgG, Alexa Fluor 647 (Invitrogen, A-31573). Amersham ECL Mouse IgG, HRP-linked F(ab')₂ fragment from sheep (GE Life Sciences, NA9310); Amersham ECL Rabbit IgG, HRP-linked F(ab')₂ fragment from donkey (GE Life Sciences, NA9340).

Bucko P., Garcia I., Manocha R., Bhat A., Wordeman L., & Scott J.D. (2020). Gravin-associated kinase signaling networks coordinate γ-tubulin organization at mitotic spindle poles.

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