MicroRNA levels were assessed by quantitative PCR (qPCR) on a CFX384 detection system (Bio-Rad) with SYBR Green using the Exiqon PCR primer sets according to manufacturer’s instructions (Exiqon Inc.). Data was normalized to reference gene expression U6 snRNA and 5S rRNA. All primers for microRNAs and the reference genes were purchased from Exiqon Inc. Quantitative PCR for cytokines and reference genes GAPDH or β-actin was performed similarly using CFX384 detection system (Bio-Rad) and SYBR Green. Primers were as noted (Supplementary Table 1 ) and data normalized to reference gene expression.
Exiqon pcr primer sets
Manufactured by Qiagen
Exiqon PCR primer sets are high-quality pre-designed primers for real-time PCR applications. These primer sets are designed to amplify specific target sequences with high efficiency and specificity.
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Lab products found in correlation
2 protocols using exiqon pcr primer sets
1
Quantitative PCR Analysis of miRNA and Cytokines
2
Quantifying microRNA and mRNA Levels
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MicroRNA expression levels were assessed by real-time polymerase chain reaction (PCR) on a CFX-384 detection system (Bio-Rad, Hercules, CA) using the Exiqon PCR primer sets according to manufacturer’s instructions (Exiqon Inc., Woburn, MA). All primers for the microRNAs and the reference genes (U6 small nuclear RNA and 5S ribosomal RNA) were purchased from Exiqon Inc. Real-time PCR (Bio-Rad) for XIAP, GAPDH and beta-actin mRNAs was performed in the same RNA samples extracted from biopsies.
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