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3 protocols using naringenin

1

Antibiotics and Naringenin Evaluation

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Naringenin was purchased from MedChemExpress (MCE) Co., Ltd. (NJ, United States). The antibiotics used in this study, including amikacin, ceftazidime, cefepime, imipenem, ciprofloxacin, aminotransim, levofloxacin, gentamicin, tobramycin, and colistin, were purchased from Wenzhou Kangtai Biotechnology Co., Ltd., Zhejiang China.
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2

Inflammatory Pathway Modulation Protocol

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Lipopolysaccharide and caerulein Meilinbio (Dalian, China). CH223191, dexamethasone (DEX), and naringenin were purchased from MedChemExpress (New Jersey, United States). NLRP3, AhR, ZO-1, and occludin antibodies were purchased from Abcam (Cambridge, United Kingdom). Antibodies against β-actin and histone H3 were purchased from BOSTER (Wuhan, China). Goat anti-rabbit IgG was purchased from Absin (Wuhan, China).
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3

Synthesis of Dihydro-coumaroyl and Coumaroyl Anthranilate Amides

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The chemical standard for methyl 3-hydroxy-2-(3-(4-hydroxyphenyl)propanamido)benzoate [dihydro-coumaroyl anthranilate amide (2)] and (E)-methyl 3-hydroxy-2-(3-(4-hydroxyphenyl)acrylamido)benzoate [coumaroyl anthranilate amide (3)] was purchased from Toronto Research Chemicals (Canada). Methyl 2-amino-3-hydroxybenzoate [3-HAA methyl ester (1)] was purchased from Apollo Scientific (UK). p-Coumaric acid, malonyl-CoA, 3-HAA, methyl 3-hydroxybenzoate, 2-amino-3-methoxybenzoic acid, 2-amino-4-methoxybenzoic acid, 2-amino-5-methoxybenzoic acid, 2-amino-5-hydroxybenzoic acid, and 2-anthranilic acid were purchased from Sigma-Aldrich (St. Louis, MO). Naringenin chalcone was purchased from Biosynth Carbosynth (USA). Naringenin was purchased from MedChemExpress (USA). p-coumaroyl-CoA standard was purchased from PlantMetaChem (Germany).
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