1 phenyl 3 methyl 5 pyrazolone

Manufactured by Sinopharm

Sourced in China

1-phenyl-3-methyl-5-pyrazolone is a chemical compound that serves as a key precursor in the synthesis of various pharmaceutical and industrial products. It is a heterocyclic organic compound with the molecular formula C10H10N2O. This substance possesses a pyrazolone core structure and is a white crystalline powder at room temperature.

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Lab products found in correlation

Anhydrous ethanol, by Tianjin Damao (3 mentions) Chloroform, by Tianjin Damao (3 mentions) N butanol, by Tianjin Damao (3 mentions) Neutral red reagent, by Solarbio (1 mentions) Chromatographic grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Glucosamine, by Merck Group (1 mentions) Sulphuric acid, by Sinopharm (1 mentions) Methyl iodide, by Sinopharm (1 mentions) Mannose, by Merck Group (1 mentions) Fucose fuc, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Dmso d6, by Merck Group (1 mentions) Sodium borohydride, by Sinopharm (1 mentions) Trifluoroacetic acid, by Sinopharm (1 mentions)

2 protocols using 1 phenyl 3 methyl 5 pyrazolone

Characterization of Polysaccharide Immunomodulatory Effects

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Dextran 180, 2500, 4600, 7100, 21,400, 41,100, 84,400, 133,800 and 2,000,000 were obtained from the National Institutes for Food and Drug Control (Beijing, China). Reference compounds, including mannose (Man), glucuronic acid, rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), arabinose (Ara), fucose (Fuc) and fructose (Fru) were acquired from Shanxi Jiujiu Trading Co., Ltd. (Taiyuan, China). Trifluoroacetic acid was obtained from China Shanghai Aladdin Biotechnology Co., Ltd. (Shanghai, China), and 1-phenyl-3-methyl-5-pyrazolone was bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Analytical methanol, anhydrous ethanol, chloroform and n-butanol were purchased from Damao Chemical Reagent Factory (Tianjin, China). Chromatographic-grade acetonitrile was obtained from Thermo Fisher (Thermo, Waltham, MA, USA). RPMI-1640 medium was supplied by Cellgro, Lincoln, NE, USA. Mouse spleen lymphocytes and mouse peritoneal macrophages were provided by Wuhan Psinuo Life Technology Co., Ltd. (Wuhan, China). Lipopolysaccharide (LPS), concanavalin (ConA), MTT and neutral red reagent were purchased from Solarbio (USA). Mouse ELISA kits for IL-2, IL-4, IFN-γ, NO, IL-6, IL-10 and TNF-α were acquired from Boster Biological Technology Co., Ltd. (Wuhan, China).

Li G., Ju Y., Wen Y., Zuo M., Wang C., Zhang X., Hou X., Yang G, & Gao J. (2022). Screening of Codonopsis radix Polysaccharides with Different Molecular Weights and Evaluation of Their Immunomodulatory Activity In Vitro and In Vivo. Molecules, 27(17), 5454.

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Characterization of Lentinus edodes Monosaccharides

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Trifluoroacetic acid (TFA), 1-phenyl-3-methyl-5-pyrazolone (PMP), phenol, sulphuric acid, alcohol, sodium borohydride (NaBH₄), and methyl iodide (CH₃I) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO); DMSO-d₆; and the monosaccharide standards mannose (Man), glucosamine (GlcN), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), galactosamine (GalN), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). All other reagents used in this study were of analytical grade and purchased from commercial vendors.
Lentinus edodes (Berk.) sing were provided by INFINITUS Company (Guangzhou, China).

Li J., Cai C., Zheng M., Hao J., Wang Y., Hu M., Fan L, & Yu G. (2019). Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes. Molecules, 24(8), 1610.

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