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2 protocols using isoniazid

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Pasakbumin A Extraction and Reagents

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Pasakbumin A was isolated from the roots of E. longifolia as previously described[44 (link)]. Rifampicin (RMP) and isoniazid (isonicotinic acid hydrazide, INH) were purchased from Tokyo Chemical Industry Co. Ltd. (TCI Co. Ltd., Tokyo, Japan). Other reagents were as follows: U0126 (Cell Signaling, Danvers, MA, USA), 3-methyladenine (3-MA, Sigma-Aldrich, St. Louis, MO, USA), and Bay11-7082 (Cayman Chemical, MI, USA).
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2

Cultivation of Mycobacterium and Actinomycetal Strains

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Kanamycin, streptomycin, amikacin, ciprofloxacin, and rifampicin were purchased from FUJIFILM Wako Pure Chemical Industries (Osaka, Japan). Clarithromycin, ethambutol, isoniazid, and pyrazinamide were purchased from Tokyo Chemical Industries (Tokyo, Japan). Unless otherwise stated, all other reagents were reagent-grade commercial products. Middlebrook 7H9 broth (Becton, Dickinson and Company, NJ, USA) was blended with 0.05% Tween 80 (Tokyo Chemical Industries) and 10% ADC enrichment [5% bovine serum albumin (FUJIFILM Wako Pure Chemical Industries), 2% glucose (FUJIFILM Wako Pure Chemical Industries), and 0.85% NaCl (FUJIFILM Wako Pure Chemical Industries)] to cultivate mycobacterium strains. Seed and production media consisted of 1.0% malt extract (Becton, Dickinson and Company), 0.4% yeast extract (Becton, Dickinson and Company), and 0.4% glucose (FUJIFILM Wako Pure Chemical Industries) to cultivate all actinomycetal strains.
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