Anti cdh1 sc 56312

The antibodies used in this study were as follows: anti-Cdh1 (sc-56312, 1:1000 WB, Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-γH2AX (05-636, 1:500 IF, 1:1000 IF, Millipore, Billerica, MA, USA); anti-BRCA1 (sc-6954, 1:200 IF, Santa Cruz); anti-Phospho-Chk1(Ser345) (2348S, 1:1000 WB, Cell Signaling, Danvers, MA, USA); anti-cleaved Caspase-3 (9664S, 1:1000 WB, Cell Signaling); anti-cleaved PARP (9664S, 1:1000 WB, Cell Signaling); anti-Actin (66009-1-Ig, 1:5000 WB, Proteintech, Wuhan, China); anti-GAPDH (60004-1-1g, 1:5000 WB, Proteintech, Wuhan, China), and anti-PICH(8886S, 1:200 IF, Cell Signaling); the secondary antibodies conjugated to horseradish peroxidase were used for Western blotting. The secondary antibodies of anti-mouse, or anti-rabbit containg Alexa Fluor 488 or 594 were used for immunofluorescence staining (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

ATO (A1010), ATRA (R2625), MG132 (M7449), Thymidine (T1895), Nocodazole (M1404), Glutathione-agarose (G4510), Carboxymethylcellulose sodium salt (CMC-Na, C4888) and Senescence Cells Histochemical Staining Kit (CS0030) were purchased from Sigma-Aldrich. Palbociclib (PD0332991, S1116) and Abemaciclib (S5716) from Selleckchem. Hoechst 33342 Solution and Dead Cell Apoptosis Kit with Annexin V FITC and PI from Thermo. Tumor Dissociation Kit (mouse) from Miltenyi Biotec. Sulfopin was provided by Dr. Nathanael Gray.
Antibodies used in this study are as follows: Anti-PIN1 mouse monoclonal antibody was provided by Dr. Xiao Zhen Zhou. Anti-Cdh1 (sc-56312) and anti-CDC20 (sc-13162) antibodies were purchased from Santa Cruz. Anti-Pin1 rabbit monoclonal antibody (ab192036), Anti-RB (ab181616), anti-APC7 (ab4171), anti-CDK4 antibody (ab68266) and anti-Thiophosphate ester (ab133473) antibodies were purchased from Abcam. Monoclonal anti-Flag M2 antibody (F1804) from Sigma. Anti-HA-Tag rabbit mAb (3724), anti-HA-Tag mouse mAb (2367), anti-CDK2 rabbit mAb (2546), anti-CDK4 (D9G3E) rabbit mAb (12790), anti-Cyclin B1 antibody (4138), anti-PLK1 rabbit mAb (4513), anti-Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (2325) and anti-Geminin rabbit mAb (52508) antibodies were purchased from Cell Signaling Technology. Anti-Emi1 mouse mAb (376600) was purchased from Thermo.