Western blot was performed as published previously12 (link), 13 (link) using E-cadherin, caspase-3, caspase-7, caspase-8, caspase-9, PARP-1, cytochrome C, XIAP, AIF, beclin-1, LC3 A/B rabbit monoclonal antibody (Cell Signaling Technology, Inc., Beverly, MA, USA), HMGCR rabbit monoclonal antibody (Abcam, UK), Vimentin, Smac/DIABLO mouse (Cell Signaling Technology, Inc., Beverly, MA, USA). Loading control was evaluated using α-tubulin rabbit monoclonal antibody (Cell Signaling Technology, Inc., Beverly, MA, USA). For evaluation of protein expression, X-ray films (GE Healthcare, Chicago, IL, USA) were scanned and analyzed by the Image StudioTM Lite 5.0 (LI-COR Biotechnology, Lincoln, NB, USA). The Integrated Density Value (IDV) was obtained as a ratio of normalized protein band densities in parental and radioresistant RR cells after background correction.
Lc3 a b rabbit monoclonal antibody
Manufactured by Cell Signaling Technology
Sourced in United States, Japan
The LC3 A/B rabbit monoclonal antibody is a laboratory tool used to detect the presence and expression levels of the LC3A and LC3B proteins. LC3 proteins are involved in the process of autophagy, a cellular mechanism for recycling and degrading damaged or unnecessary cellular components. This antibody can be used in various analytical techniques, such as Western blotting and immunohistochemistry, to study the role of LC3 proteins in biological processes.
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Lab products found in correlation
Image studio lite 5, by LI COR (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
X ray film, by GE Healthcare (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
α tubulin rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Parp1, by Cell Signaling Technology (1 mentions)
Bcl 2 rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Bax rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Enhanced chemiluminescence detection system, by Cytiva (1 mentions)
2 protocols using lc3 a b rabbit monoclonal antibody
1
Western Blot Analysis of Apoptosis-related Proteins
2
Western Blot Analysis of Apoptosis Markers
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The cell lysates (10 μl/lane) were separated on a polyacrylamide gel membrane.
After the nonspecific binding sites were blocked with 3 % skim milk, the membrane was treated overnight with Bax Rabbit monoclonal antibody, Bcl‐2 Rabbit monoclonal antibody and LC3A/B Rabbit monoclonal antibody (diluted 1:1000; Cell Signaling Japan). The immunoreactive bands were demonstrated by incubation with anti‐Rabbit IgG‐HRP (IBL) at room temperature for 1 h. Peroxidase activity was visualized with the enhanced chemiluminescence detection system (Amersham). Integrated optical intensities of the immunoreactive protein bands were quantified by imaging and the analysis software Multi Gauge; they were normalized to GAPDH values.
After the nonspecific binding sites were blocked with 3 % skim milk, the membrane was treated overnight with Bax Rabbit monoclonal antibody, Bcl‐2 Rabbit monoclonal antibody and LC3A/B Rabbit monoclonal antibody (diluted 1:1000; Cell Signaling Japan). The immunoreactive bands were demonstrated by incubation with anti‐Rabbit IgG‐HRP (IBL) at room temperature for 1 h. Peroxidase activity was visualized with the enhanced chemiluminescence detection system (Amersham). Integrated optical intensities of the immunoreactive protein bands were quantified by imaging and the analysis software Multi Gauge; they were normalized to GAPDH values.
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