Ym201636

RPMI 1640 and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Dimethylsulfoxide (DMSO), Hoechst 33342, XAV-939, Gefitinib and YM201636 were purchased from Sigma (St. Louis, MO, USA). U0126 was purchased from Cell Signaling Technology (Beverly, MA, USA). Polyclonal anti-human β-catenin antibody, monoclonal anti-human EGFR antibody, monoclonal anti-human phospho-EGFR (Y¹⁰⁶⁸) antibody, monoclonal anti-human β-actin antibody and the corresponding horseradish peroxidase-conjugated second antibodies were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). Monoclonal anti-human EEA.1, monoclonal anti-human phospho-ERK_1/2 (Thr²⁰²) antibody, polyclonal anti-human ERK_1/2 and monoclonal anti-human Lamin B antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). The secondary anti-mouse or anti-rabbit antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 568 was purchased from Invitrogen (Carlsbad, CA, USA). Two different Akt1 specific siRNAs purchased from GE Dharmacon (Lafayette, CO, USA) were used: ACA AGG ACG GGC ACA TTAA (1^#siRNA), CAA GGG CAC TTT CGG CAAG (2^#siRNA).

Spermine, N1‐acetylspermidine, N8‐acetylspermidine, bafilomycin A1, nocodazole, BAPTA‐AM and YM201636 were purchased from Sigma‐Aldrich (St. Louis, MO). N1‐AcetylSpermine, N1, N12‐diacetylSpermine, N1, N8‐diacetylspermidine, acrolein and hydrogen peroxide were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). Aminoguanidine hydrochloride was from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Ciliobrevin D, Jak3 inhibitor VI and Gö 6976 were from Merck Millipore (Burlington, MA).