Blood (1 ml) was collected using a tiger top 3.5 ml serum separator tube (BD Vacutainer SST Plus Blood Collection Tube). Samples were allowed to clot upright at room temperature for 30 min in processing lab (45 ± 15 min from time of collection), then spun at 1200 RCF (g) at room temperature for 15 min if fixed angle centrifuge rotor. Spun serum was then collected and transferred using a disposable transfer pipette into a 2 ml cyrovials with red cap inserts (USA Scientific REF 1420-9705). A fiberboard cryogenic storage box (Fisher Part No. 03-395-114 or equivalent) was used to store spun serum aliquots. The samples were then stored in −80°C freezer. The samples were stored (until all samples were collected) and then hand-carried in dry ice, from our laboratory to Banyan Biomarkers to be process immediately upon arrival.
Vacutainer sst plus blood collection tube
Manufactured by BD
The BD Vacutainer SST Plus Blood Collection Tube is a laboratory specimen collection device used to collect and process blood samples. It is designed to separate the serum from the cellular components of the blood through centrifugation.
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Lab products found in correlation
4 protocols using vacutainer sst plus blood collection tube
1
Blood Sample Collection and Storage
2
Neonatal Serum Collection and Storage
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Serum samples (1 ml) were collected using a 3.5 ml serum separator tube (SST, BD Vacutainer® SST Plus Blood Collection Tube, Franklin Lakes, NJ). Samples were allowed to clot in an upright position at room temperature for 30 min in the processing lab (45 ± 15 min from time of collection), then centrifuged at 1,200 g at room temperature for 15 min in a fixed angle centrifuge rotor. Immediately following centrifugation, the serum was transferred from the SST using a disposable transfer pipette into a 2 ml cryovial with a red cap insert (USA Scientific, Orlando, FL). The serum samples were stored at 4 °C. A fiberboard cryogenic storage box (Fisher Scientific, Pittsburgh, PA) was used to store serum aliquots at −80°C until assay analysis. Blood collection from neonates was completed in accordance with common practice as well as state and federal regulations.
3
Coronary Blood Sampling Protocol
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Coronary blood was withdrawn from the coronary root at the beginning of the cardiac catheterization protocol and the end of revascularization. Blood was transferred into BD Vacutainer SST Plus Blood Collection Tubes, placed on ice, and then centrifuged at 1,500 g for 20 min. Plasma was stored at−80°C until use.
4
Biobanking of Blood and Tumor Samples
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The patient was provided written consent and enrolled and all samples used in this study were collected in accordance with the Institutional Review Board of the Icahn School of Medicine at Mount Sinai (GCO# 10-1166). All experimental protocols were approved by the Icahn School of Medicine at Mount Sinai in accordance with our research protocol (GCO# 10-1166). All clinical investigations were conducted according to the principles expressed in the Declaration of Helsinki. Patient samples were collected over a 2-yr period between 2017 and 2019.
Blood and tumor samples were collected and biobanked at the Icahn School of Medicine at Mount Sinai. For plasma separation, blood samples were collected in BD Vacutainer SST Plus Blood Collection Tubes (BD Biosciences) and processed between 4 and 6 h after collection. Blood samples were centrifuged at 1200 rpm for 10 min at 4°C for separation of plasma. All samples were aliquoted and stored at −130°C until use.
Blood and tumor samples were collected and biobanked at the Icahn School of Medicine at Mount Sinai. For plasma separation, blood samples were collected in BD Vacutainer SST Plus Blood Collection Tubes (BD Biosciences) and processed between 4 and 6 h after collection. Blood samples were centrifuged at 1200 rpm for 10 min at 4°C for separation of plasma. All samples were aliquoted and stored at −130°C until use.
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