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2 protocols using 2 4 6 tris 2 pirydyl s triazine

1

Antioxidant Activity of Tannin Extracts

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Chestnut tannins (CT) and quebracho tannins (QT) were provided by Silvateam (S. Michele Mondovì, Cuneo, Italy).
Whatman® Nylon membrane filters (0.45 μm pore size, 47 mm diameter) were from Sigma-Aldrich (Milan, Italy).
2,2-Diphenyl-1-picrylhydrazyl (DPPH), iron (III) chloride hexahydrate (97%), iron (II) chloride tetrahydrate, iron (II) sulfate, 2,4,6-tris(2-pirydyl)-s-triazine (TPTZ) (≥98%), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) (97%), sodium borohydride, and ethylenediaminetetraacetic acid (EDTA) sodium salt were obtained from Sigma-Aldrich (Milan, Italy) and used as obtained.
The laccase used in this study was the recombinant POXA1b from the fungus Pleurotus ostreatus heterologously produced in the yeast Pichia pastoris [28 (link)].
UV-Vis spectra were recorded using a HewlettPackard 8453 Agilent spectrophotometer.
Chemical structures were drawn with the software ChemDraw Ultra 12.0.
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2

Antioxidant Capacity Evaluation Protocol

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Horseradish peroxidase (HRP), 30% hydrogen peroxide, 2,2-diphenyl-1-picrylhydrazyl (DPPH), iron (III) chloride (97%), 2,4,6-tris(2-pirydyl)-s-triazine (TPTZ) (≥98%), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) (97%), ethylenediaminetetraacetic acid (EDTA) (>99%), nitroblue tetrazolium (NBT) chloride (98%), sodium nitroprusside (SNP), N-(1-naphthyl)ethylenediamine dihydrochloride, sulphanilamide, gallic acid (≥97.5%), pyrogallol, catechol, 4-methylcatechol, caffeic acid, ferulic acid, p-coumaric acid, vanillic acid, tyrosol and quercetin were obtained from Sigma-Aldrich (Milan, Italy).
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