Whatman® Nylon membrane filters (0.45 μm pore size, 47 mm diameter) were from Sigma-Aldrich (Milan, Italy).
2,2-Diphenyl-1-picrylhydrazyl (DPPH), iron (III) chloride hexahydrate (97%), iron (II) chloride tetrahydrate, iron (II) sulfate, 2,4,6-tris(2-pirydyl)-s-triazine (TPTZ) (≥98%), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) (97%), sodium borohydride, and ethylenediaminetetraacetic acid (EDTA) sodium salt were obtained from Sigma-Aldrich (Milan, Italy) and used as obtained.
The laccase used in this study was the recombinant POXA1b from the fungus Pleurotus ostreatus heterologously produced in the yeast Pichia pastoris [28 (link)].
UV-Vis spectra were recorded using a HewlettPackard 8453 Agilent spectrophotometer.
Chemical structures were drawn with the software ChemDraw Ultra 12.0.