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Sensolyte plus 520 mmp 2 assay kit

Manufactured by AnaSpec
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The SensoLyte® Plus 520 MMP-2 Assay Kit is a fluorometric assay designed for the detection and quantification of matrix metalloproteinase-2 (MMP-2) activity. The kit utilizes a specific MMP-2 substrate that, upon cleavage, generates a fluorescent signal. This allows for the measurement of MMP-2 activity in biological samples.

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Lab products found in correlation

Sensolyte plus 520 mmp 13 assay kit, by AnaSpec (1 mentions) Sensolyte 520 mmp14 assay kit, by AnaSpec (1 mentions)

Close spelling variants of this product

SensoLyte 520 MMP-2 Assay Kit (6 citations) SensoLyte Plus 520 (2 citations) MMP-2 (5 citations)

2 protocols using sensolyte plus 520 mmp 2 assay kit

1

MMP Activity Assay Protocol

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Culture supernatants were collected 7 days after the IC-2 treatment, and cells were homogenized in the lysis buffer provided with the following assay kits: SensoLyte® 520 MMP-1 Assay Kit ∗Fluorimetric∗, SensoLyte® 520 MMP-14 Assay Kit, SensoLyte® Plus 520 MMP-2 Assay Kit, and SensoLyte® Plus 520 MMP-13 Assay Kit (AnaSpec, Inc., CA, USA). Samples were stored at −80 °C until analysis, and each MMP activity was measured according to the manufacturer's instructions.
Fukushima K., Itaba N., Kono Y., Okazaki S., Enokida S., Kuranobu N., Murakami J., Enokida M., Nagashima H., Kanzaki S., Namba N, & Shiota G. (2021). Secreted matrix metalloproteinase-14 is a predictor for antifibrotic effect of IC-2-engineered mesenchymal stem cell sheets on liver fibrosis in mice. Regenerative Therapy, 18, 292-301.
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2

Quantifying MMP2 Activity in HK-2 Cells

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At 70% confluency, the complete media was replaced with medium containing 1× ITS media supplement and incubated overnight. The quiescent HK-2 cells were then incubated with HG (25 mM) for 12 h. MMP2 activity in equal amounts of culture supernatants was analyzed according to manufacturer’s instructions using a SensoLyte Plus 520 MMP2 Assay Kit *Fluorometric and Enhanced Sensitivity*(#AS-72224; AnaSpec, Fremont, CA). Following immunocapture of MMP2 by immobilized anti-MMP-2 antibody, its proteolytic activity was measured by 5-FAM/QXL® 520 FRET peptide substrate by monitoring the fluorescent signal at Ex/Em = 490/520 nm.
Das N.A., Carpenter A.J., Belenchia A., Aroor A.R., Noda M., Siebenlist U., Chandrasekar B, & DeMarco V.G. (2019). Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-tomesenchymal transition. Cellular signalling, 68, 109506.
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