To determine the mechanism underlying the inhibitory effect of BRD9 inhibition on the uLMS, the SK-UT-1 cells were treated with iBRD9 TP-472 (5 µM) for 48 h. RNA was isolated using Trizol. RNA quality and quantity were assessed using the Agilent bio-analyzer. Strand-specific RNA-SEQ libraries were prepared using a TruSEQ total RNA-SEQ library protocol (Illumina provided). Library quality and quantity were assessed using the Agilent bio-analyzer and libraries were sequenced using an Illumina NovaSEQ6000 (illumine provided reagents and protocols).
Truseq total rna seq library protocol
Manufactured by Illumina
Sourced in United States
The TruSEQ total RNA-SEQ library protocol is a laboratory workflow designed to prepare samples for RNA sequencing. It enables the construction of libraries from total RNA samples, including both coding and non-coding RNA species. The protocol includes steps for RNA extraction, fragmentation, cDNA synthesis, adapter ligation, and library amplification.
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Lab products found in correlation
2 protocols using truseq total rna seq library protocol
1
Elucidating Mechanism of BRD9 Inhibition in uLMS
2
Mechanism of BRD9 Inhibition in UFs
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To determine the mechanism underlying the inhibitory effect of BRD9 inhibition on the UFs, the UF HuLM cells were treated with BRD9 inhibitor I-BRD9 (5 µM, n = 4) and DMSO vehicle control (n = 4) for 48 h. RNA was isolated using Trizol. RNA quality and quantity were assessed using the Agilent bio-analyzer. Strand-specific RNA-SEQ libraries were prepared using a TruSEQ total RNA-SEQ library protocol (Illumina provided, San Diego, CA, USA). Library quality and quantity were assessed using the Agilent bio-analyzer, and libraries were sequenced using an Illumina NovaSEQ6000 (illumine provided reagents and protocols).
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