Purified mouse ab anti human cd3

Manufactured by BD

Sourced in Italy

Purified mouse ab anti-human CD3 is a monoclonal antibody that specifically binds to the CD3 complex on human T cells. It is a common tool used in research applications.

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Lab products found in correlation

Heat inactivated fetal bovine serum, by Euroclone (2 mentions) Penicillin streptomycin, by Euroclone (2 mentions) Purified mouse ab anti human cd28, by BD (2 mentions) Rpmi 1640, by Euroclone (2 mentions) Dopamine hydrochloride, by Merck Group (2 mentions) Ficoll paque plus, by GE Healthcare (2 mentions) Pd 168 077 maleate, by Merck Group (1 mentions) Skf 38393 hydrochloride, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Tgf β, by Miltenyi Biotec (1 mentions) Il 12, by Miltenyi Biotec (1 mentions) Il 1β, by Miltenyi Biotec (1 mentions) Anti il 4, by Miltenyi Biotec (1 mentions) Phytohaemagglutinin pha, by Merck Group (1 mentions) Interleukin 6 (il 6), by Miltenyi Biotec (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) Interleukin 4 (il 4), by Miltenyi Biotec (1 mentions)

2 protocols using purified mouse ab anti human cd3

In Vitro Immune Cell Activation

Cited 1 time

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Bovine serum albumin (BSA) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Sigma, Italy. RPMI 1640, heat-inactivated fetal bovine serum (FBS), glutamine, and penicillin/streptomycin were obtained from Euroclone, Italy. Ficoll-Paque Plus was from Pharmacia Biotech (Uppsala, Sweden). Purified mouse ab anti-human CD3 (code 555330, clone UCHT1, Mouse IgG1, κ) and purified mouse ab anti-human CD28 (code 555726, clone CD28.2, Mouse IgG1, κ) were obtained from Becton Dickinson, Italy. (±)SKF-38,393 hydrochloride (cod. D047), R(+)7-OH-DPAT hydrobromide (code H168), PD-168,077 maleate (code P233), pramipexol dihydrochloride (code A1237), and dopamine hydrochloride (code H8502) were all from Sigma, Italy. Human recombinant α-synuclein and its fibrillar form were a kind gift from Dr. Lars Kjær and Dr. Daniel Otzen (iNANO - Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark), and were prepared as published before55 (link).

Kustrimovic N., Rasini E., Legnaro M., Bombelli R., Aleksic I., Blandini F., Comi C., Mauri M., Minafra B., Riboldazzi G., Sanchez-Guajardo V., Marino F, & Cosentino M. (2016). Dopaminergic Receptors on CD4+ T Naive and Memory Lymphocytes Correlate with Motor Impairment in Patients with Parkinson’s Disease. Scientific Reports, 6, 33738.

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Cytokine Production Assay Protocol

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Bovine serum albumin (BSA) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Sigma, Italy. RPMI 1640, heat-inactivated fetal bovine serum (FBS), glutamine, and penicillin/streptomycin were obtained from Euroclone, Italy. Ficoll-Paque Plus was from Pharmacia Biotech (Uppsala, Sweden, code GEH1714403). Purified mouse ab anti-human CD3 (code 555330, clone UCHT1, Mouse IgG1, κ) and purified mouse ab anti-human CD28 (code 555726, clone CD28.2, Mouse IgG1, κ) were obtained from Becton Dickinson, Italy. Phytohaemagglutinin (PHA, code L8902), recombinant interleukin (IL)-2 (code 0208AF12), dopamine hydrochloride (code H8502), phorbol 12-myristate 13-acetate (PMA, code P813), and ionomycin (code I3909) were all from Sigma-Aldrich (Saint Louis, MO, USA).
Human ab anti-INF-γ (code 130-095-743) and anti-IL-4 (code 130-095-753), IL-1β (code 130-093-895), IL-4 (code 130-095-373), IL-6 (code 130-095-365), IL-12 (code 130-096-704), and TGF-β (code 130-095-067) were from Miltenyi Biotec, Bergisch Gladbach, Germany.
ELISA kits for human interferon (IFN)-γ (code EHIFNG), tumor necrosis factor (TNF)-α (code EH3THFA), IL-4 (code EHIL4), IL-10 (code EHIL10), and IL-17A (code EHIL17A) were all from Thermo Scientific, Rockford, USA.

Kustrimovic N., Comi C., Magistrelli L., Rasini E., Legnaro M., Bombelli R., Aleksic I., Blandini F., Minafra B., Riboldazzi G., Sturchio A., Mauri M., Bono G., Marino F, & Cosentino M. (2018). Parkinson’s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naïve and drug-treated patients. Journal of Neuroinflammation, 15, 205.

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