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Anti phosphorylated akt ps473

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Anti phosphorylated AKT (pS473) is a primary antibody that specifically recognizes the serine 473 phosphorylated form of the AKT protein. AKT is a key regulator of cellular processes such as cell growth, proliferation, and survival. This antibody can be used to detect and quantify the phosphorylation status of AKT in various experimental models.

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Lab products found in correlation

Cofilin, by Santa Cruz Biotechnology (2 mentions) Slowfade gold antifade mountant with dapi, by Thermo Fisher Scientific (2 mentions) Lsm 710 confocal laser scanning microscope, by Zeiss (2 mentions) Anti cortactin mab 4f11, by Merck Group (2 mentions) Alexa fluor 568 labeled goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (2 mentions) Sc 8350, by Santa Cruz Biotechnology (2 mentions) Nck1 ab23120, by Abcam (2 mentions) Alexa fluor 488 labeled goat anti rabbit igg secondary antibody, by Thermo Fisher Scientific (2 mentions)

2 protocols using anti phosphorylated akt ps473

1

Immunofluorescence Analysis of Cortactin and Kinases

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Cells were seeded in 5% DMEM-F12 medium with 5% horse serum overnight and treated with 0.2 ng ml−1 EGF for three hours prior to fixation with 4% paraformaldehyde. Fixed cells were permeabilized with 0.05% Triton X-100 and blocked with 3% bovine serum albumin in phosphate-buffered saline. Cortactin was visualized with by immunofluorescence (IF) with anti-cortactin mAb 4F11 (05-180; 1:500) from Millipore and Alexa Fluor 568 labeled goat anti-mouse IgG secondary antibody (A11031; 1:1,000) from Invitrogen. IF assays were also performed with rabbit monoclonal anti phosphorylated AKT (pS473) (4058: 1:200) antibody from Cell Signaling Technology and rabbit polyclonal antibodies against WASP (SC-8350: 1:250) from Santa Cruz, NCK1 (ab23120: 1:200) from Abcam and cofilin (SC-33779:1:200) from Santa Cruz followed Alexa fluor 488-labeled goat anti rabbit IgG secondary antibody (A11034, 1:1,000) from Invitrogen. The nuclei were stained with SlowFade Gold Antifade Mountant with DAPI (S36938) from Life Technologies. Immunofluorescence analysis was carried out using a LSM710 confocal laser scanning microscope (Carl Zeiss).
Wu X., Renuse S., Sahasrabuddhe N.A., Zahari M.S., Chaerkady R., Kim M.S., Nirujogi R.S., Mohseni M., Kumar P., Raju R., Zhong J., Yang J., Neiswinger J., Jeong J.S., Newman R., Powers M.A., Somani B.L., Gabrielson E., Sukumar S., Stearns V., Qian J., Zhu H., Vogelstein B., Park B.H, & Pandey A. (2014). Activation of diverse signaling pathways by oncogenic PIK3CA mutations. Nature communications, 5, 4961.
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2

Immunofluorescence Analysis of Cortactin and Kinases

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded in 5% DMEM-F12 medium with 5% horse serum overnight and treated with 0.2 ng ml−1 EGF for three hours prior to fixation with 4% paraformaldehyde. Fixed cells were permeabilized with 0.05% Triton X-100 and blocked with 3% bovine serum albumin in phosphate-buffered saline. Cortactin was visualized with by immunofluorescence (IF) with anti-cortactin mAb 4F11 (05-180; 1:500) from Millipore and Alexa Fluor 568 labeled goat anti-mouse IgG secondary antibody (A11031; 1:1,000) from Invitrogen. IF assays were also performed with rabbit monoclonal anti phosphorylated AKT (pS473) (4058: 1:200) antibody from Cell Signaling Technology and rabbit polyclonal antibodies against WASP (SC-8350: 1:250) from Santa Cruz, NCK1 (ab23120: 1:200) from Abcam and cofilin (SC-33779:1:200) from Santa Cruz followed Alexa fluor 488-labeled goat anti rabbit IgG secondary antibody (A11034, 1:1,000) from Invitrogen. The nuclei were stained with SlowFade Gold Antifade Mountant with DAPI (S36938) from Life Technologies. Immunofluorescence analysis was carried out using a LSM710 confocal laser scanning microscope (Carl Zeiss).
Wu X., Renuse S., Sahasrabuddhe N.A., Zahari M.S., Chaerkady R., Kim M.S., Nirujogi R.S., Mohseni M., Kumar P., Raju R., Zhong J., Yang J., Neiswinger J., Jeong J.S., Newman R., Powers M.A., Somani B.L., Gabrielson E., Sukumar S., Stearns V., Qian J., Zhu H., Vogelstein B., Park B.H, & Pandey A. (2014). Activation of diverse signaling pathways by oncogenic PIK3CA mutations. Nature communications, 5, 4961.
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