Testosterone (>98%) and finasteride (>98%) were obtained from Tokyo Chemical Industry (Kita-ku, Tokyo, Japan). Linoleic acid and β-sitosterol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against β-catenin, Lef-1, TGF-β, Cyclin E, Smo, Shh, β-actin and peroxidase-conjugated antigoat were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cyclin D1, Bcl-2, Bax, and peroxidase-conjugated antirabbit antibodies were obtained from Cell Signaling Technology (Boston, MA, USA). Gli-1 antibody was purchased from Abcam (Cambridge, UK). BICYCLO was provided from the Laboratory of Synthetic Pharmaceutical Chemistry at Inje University (Gimhae, Korea). All other reagents used in this study were of the highest grade commercially available.
Gli 1 antibody
Manufactured by Abcam
Sourced in United Kingdom
The Gli-1 antibody is a protein-specific reagent used to detect the expression of the Gli-1 transcription factor. Gli-1 is a key mediator of the Hedgehog signaling pathway, which plays a role in embryonic development and cancer progression.
Automatically generated - may contain errors
Lab products found in correlation
Testosterone, by Tokyo Chemical Industry (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Peroxidase conjugated anti goat, by Santa Cruz Biotechnology (1 mentions)
Finasteride, by Tokyo Chemical Industry (1 mentions)
Antibodies against β catenin, by Santa Cruz Biotechnology (1 mentions)
Cyclin e, by Santa Cruz Biotechnology (1 mentions)
β sitosterol, by Merck Group (1 mentions)
Tgf β, by Santa Cruz Biotechnology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Tnf α, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
Smad4, by Proteintech (1 mentions)
Trypsin edta, by Sartorius (1 mentions)
Fetal calf serum fbs, by Sartorius (1 mentions)
Smad2, by Proteintech (1 mentions)
β actin, by Proteintech (1 mentions)
α sma, by Proteintech (1 mentions)
2 protocols using gli 1 antibody
1
Investigating Molecular Mechanisms in Preclinical Research
2
Transgenic Mouse Model for Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Green fluorescence transgenic male C57Bl/6 mice (n = 20) and normal male C57Bl/6 mice (n = 36) 2343 bought from Gem Pharmatech Biotechnology Co., LTD (Jiangsu, China), with certificate No.320727210100567527. The DMEM high-sugar medium, fetal calf serum (FBS), penicillin-streptomycin, phosphate buffer (PBS), trypsin–EDTA were bought from Biological Industries (Israel). The Gli1 antibody was bought from Abcam (Cambridge, England). α-SMA, SMAD2, SMAD4, β-actin and goat anti-rabbit IgG II were bought from Proteintech Group Inc. (Rosemont, USA). The antibodies of CD44, CD177 and CD34 were bought from Cell Signaling Technology, Inc. (Boston, USA); TNF-a. IL-1, IL-1B, BUN and Scr ELISA kit were bought from Bluef (Shanghai, China) Biotechnology Development Co., LTD. (Shanghai, China). The apoptosis detection kit was bought from Beyotime Biotechnology (Shanghai, China).
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