Phospho hsp27 ser82

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-HSP27 (Ser82) is a lab equipment product that detects phosphorylation of the Ser82 residue on the HSP27 protein. HSP27 is a small heat shock protein involved in cellular stress response.

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Lab products found in correlation

Phospho p38 mapk thr180 tyr182, by Cell Signaling Technology (2 mentions) Phospho c jun ser73, by Cell Signaling Technology (1 mentions) Phospho sapk jnk thr183 tyr185, by Cell Signaling Technology (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Gapdh, by Abcam (1 mentions) β actin, by Abcam (1 mentions) P erk, by Santa Cruz Biotechnology (1 mentions) Control sirna, by Qiagen (1 mentions) Sensifast sybr no rox kit, by Meridian Bioscience (1 mentions) Hiperfect transfection, by Qiagen (1 mentions) Anti gapdh, by Merck Group (1 mentions) γ 33p atp, by Hartmann Analytic (1 mentions)

Close spelling variants of this product

HSP27 (39 citations) Phospho-HSP27 (16 citations) Anti-phospho-HSP27 (Ser82) (4 citations) Rabbit α-HSP27 phospho-Ser82 (2 citations)

3 protocols using phospho hsp27 ser82

Antioxidant Enzyme and Oxidative Stress Protocol

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The chemicals used in this study were purchased from the following sources: rabbit monoclonal catalase (MAB-94599), SOD-1 (MAB-94600), SOD-2 (MAB-94601), Cleaved-Caspase-3 (D175) (AB-84283), mouse monoclonal GPx1 (MAB-94602) and ECL substrate (Pierce ECL-2001) from Immunological Sciences. Rabbit monoclonal Phospho-HSP27 (Ser82) (#9709), Phospho-c-Jun (Ser73) (#3270), Phospho-MAPKAPK-2 (Thr334) (#3007), Phospho-p38 MAPK (Thr180/Tyr182) (#4511), Phospho-SAPK/JNK (Thr183/Tyr185) (#4668), secondary antibodies goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP; #7074) and horse anti-mouse IgG conjugated to horseradish peroxidase (HRP; #7076) from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal DNA polymerase γ (sc-390634), Cleaved-Caspase-7 (sc-56063), Bax (sc-7480) and rabbit polyclonal actin (sc-1616) from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Exgene^TM Genomic DNA Micro (cat. GA-118-050) was obtained from Geneall Biotechnology Co. Ltd. (Seoul, Republic of Korea) and Mouse Real-Time PCR Mitochondrial DNA Damage Analysis Kit (cat. DD2M) was purchased from Detroit R&D. All of the other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), and were of the purest analytical grade.

Di Nisio V., Antonouli S., Colafarina S., Zarivi O., Rossi G., Cecconi S, & Poma A.M. (2023). Repeated Rounds of Gonadotropin Stimulation Induce Imbalance in the Antioxidant Machinery and Activation of Pro-Survival Proteins in Mouse Oviducts. International Journal of Molecular Sciences, 24(11), 9294.

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Western Blot Analysis of Signaling Pathways

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Cells were washed in PBS and then lysed in RIPA lysis buffer containing protease and phosphatase inhibitors. 40 μg of protein was separated by 12% SDS-polyacrylamide electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). Membranes were blocked with 5% non-fat milk in PBST (containing 0.1% Tween-20) for 1 h, and incubated with antibodies specific to Phospho-p38 MAPK (Thr180/Tyr182) (1:1000; Cell Signaling), pERK (1:200; Santa Cruz), ERK2 (1:200; Santa Cruz), Phospho-HSP27 (Ser82) (1:1000; Cell Signaling), β-actin (1:5000; Abcam) and GAPDH (1:2500; Abcam) for ON at 4°C. Membranes were washed with PBS-T for three times followed by 1h incubation with appropriate secondary antibodies, and then visualized using the odyssey imaging system (LI-COR).

Witt-Kehati D., Fridkin A., Alaluf M.B., Zemel R, & Shlomai A. (2018). Inhibition of pMAPK14 Overcomes Resistance to Sorafenib in Hepatoma Cells with Hepatitis B Virus. Translational Oncology, 11(2), 511-517.

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Immunoblotting antibody panel for cell signaling

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Antibodies against Ras, ERK3, p53, phospho-p53 (S15 (9284), S37 (9289) and S46 (2521)) and phospho-Hsp27 (Ser82) were obtained from Cell Signaling Technology, anti-PRAK antibody from Sigma and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) antibody from Millipore. Anti-GFP (B2), anti-GST (B-14), anti-EF-2 (C-14), Hsp27 (C-20) and secondary antibodies were purchased from Santa Cruz Biotechnology. Control siRNA (Allstars negative control), HiPerFect transfection and TopTaq DNA polymerase reagent were obtained from Qiagen, mouse MK5/PRAK siRNA (sc-36311) from Santa Cruz Biotechnology, [γ-³³P]ATP from Hartmann Analytic, LMP Agarose from Bethesda Research Laboratories and SensiFAST SYBR No-Rox Kit and Vybrant DyeCycle Ruby Stain from Bioline.

Ronkina N., Johansen C., Bohlmann L., Lafera J., Menon M.B., Tiedje C., Laaß K., Turk B.E., Iversen L., Kotlyarov A, & Gaestel M. (2015). Comparative Analysis of Two Gene-Targeting Approaches Challenges the Tumor-Suppressive Role of the Protein Kinase MK5/PRAK. PLoS ONE, 10(8), e0136138.

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