Secondary irdye labeled goat anti mouse and anti rabbit igg antibodies

The following antibodies were used for Western blotting. A mouse hybridoma clone expressing mAb 15A6 recognizing an epitope in the C-terminal CHR domain of PCSK9 was a generous gift from J. Horton (University of Texas Southwestern Medical Center, Dallas, TX). The IgG fraction was purified from hybridoma culture medium by Protein A chromatography on a Profinia^TM protein purification system (Bio-Rad) as per the manufacturer's instructions. Rabbit antiserum 3143 against the C-terminal 14 amino acids of LDLR was a kind gift of J. Herz (University of Texas Southwestern Medical Center). Anti-actin mouse monoclonal ascites fluid (clone AC-40) was from Sigma. The mouse anti-human transferrin receptor antibody was from Life Technologies, Inc. Secondary IRDye-labeled goat anti-mouse and anti-rabbit IgG antibodies were from LI-COR Biosciences. Rabbit antiserum 1697 raised against full-length human PCSK9 was used for immunoprecipitation and was custom-produced by Biomatik (Cambridge, Canada).

rabbit anti-serum 3143 against the C-terminal 14 amino acids of the LDLR was the kind gift of J. Herz (University of Texas Southwestern Medical Center, Dallas, TX); C7 antibody was purified from conditioned medium of mouse hybridoma cells (ATCC, CRL-1691) by protein A affinity chromatography using Profinia^TM affinity chromatography purification system (Bio-Rad); mouse anti-human transferrin receptor antibody was purchased from Life Technologies; monoclonal anti-FLAG M2 antibody were from Sigma-Aldrich. Secondary IRDye-labeled goat anti-mouse and anti-rabbit IgG antibodies were from LI-COR Biosciences.