Super-resolution images were acquired with a Leica TCS SP8 X White Light Laser confocal microscope with Hybrid spectral detectors and HyVolution (Leica Microsystems, Wetzlar, Germany) using the Leica LAS X software (version 3.1.5). Images were acquired using 100× or 63× oil immersion objectives. The original data was stored as 16-bit greyscale images with a spatial resolution of 1,024 × 1,024 pixels. Z-stacks were acquired every 0.16 μm along with the cell thickness. Image processing and analysis were performed using Image J/Fiji software (NIH, US National Institutes of Health) and the Leica Application Suite X (LAS-X) software (Leica Microsystems). To compare the data, identical settings were used for image acquisition of different experiments and negative control samples were used for background setting previous to image acquisition.
Hybrid spectral detectors and hyvolution
Manufactured by Leica Microsystems
Sourced in Germany
Hybrid spectral detectors and HyVolution are specialized imaging technologies offered by Leica Microsystems. Hybrid spectral detectors combine multiple detection channels to capture comprehensive spectral information. HyVolution is a superresolution imaging technique that enhances spatial resolution without compromising sensitivity.
Automatically generated - may contain errors
3 protocols using hybrid spectral detectors and hyvolution
1
Super-resolution Confocal Microscopy Protocol
2
Confocal Microscopy Super-resolution Imaging
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Super-resolution images were acquired with a Leica TCS SP8 X White Light Laser confocal microscope with Hybrid spectral detectors and HyVolution (Leica Microsystems, Wetzlar, Germany) using the Leica LAS X software (version 3.1.5). Image deconvolution was performed with Huygens Essential software v.4.4 0p6 (SVI, Leiden, The Netherlands). Image processing and analysis were performed using the Leica Application Suite X (LAS-X) software (Leica Microsystems, Wetzlar, Germany) or ImageJ (NIH, http://rsb.info.nih.gov/ij ).
3
Super-resolution Imaging and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Super-resolution images were acquired with a Leica TCS SP8 X White Light Laser confocal microscope with Hybrid spectral detectors and HyVolution (Leica Microsystems, Wetzlar, Germany) using the Leica LAS X software (version 3.1.5). Image deconvolution was performed with Huygens Essential software v 4.4 0p6 (SVI, Leiden, The Netherlands). Image processing and analysis were implemented and performed using the software MATLAB R_2018a (The MathWorks Inc., Natick, MA, USA) and the Leica Application Suite X (LAS-X) software (Leica Microsystems, Wetzlar, Germany).
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