Coronary arterioles were embedded in OCT compound (Tissue-Tek; Electron Microscopy Sciences, Hatfield, PA, USA) and frozen sections (10 μm thickness) were fixed in 4% paraformaldehyde for immunohistochemical analysis as described previously [83 (link),84 (link)]. Immunolabelling was performed using a mouse monoclonal antibody against eNOS (610297, 1:100 dilution; BD Biosciences, Franklin Lakes, NJ, USA) and a rabbit polyclonal antibody against PKCβ2 (sc-210, 1:100 dilution; Santa Cruz Biotechnology, Dallas, TX, USA). The slides were then incubated with rhodamine red-labeled (Jackson Laboratories, West Grove, PA, USA) and FITC-labeled (Jackson Laboratories) secondary antibodies. Staining control tissues were exposed for the same duration to non-immune serum (Jackson Laboratories) in place of primary antibody. Slides were observed for red (rhodamine red for PKCβ2) and green (FITC for eNOS) images under a fluorescence microscope (Axiovert 200, Zeiss, Jena, Germany) and analyzed using ImageJ software (National Institutes of Health, Bethesda, MD, USA) as described previously [20 (link)].
Rhodamine red
Manufactured by Zeiss
Sourced in Germany
Rhodamine red is a fluorescent dye commonly used in various laboratory applications. It is characterized by its bright red-orange emission spectrum when excited by appropriate light sources. The dye is known for its high quantum yield and photostability, making it a valuable tool for fluorescence-based techniques and imaging.
Automatically generated - may contain errors
Lab products found in correlation
Fitc labeled, by Jackson ImmunoResearch (2 mentions)
Rhodamine red labeled, by Jackson ImmunoResearch (2 mentions)
Non immune serum, by Jackson ImmunoResearch (2 mentions)
Axiovert 200, by Zeiss (2 mentions)
Fluorescein isothiocyanate (fitc), by Zeiss (1 mentions)
Tissue tek, by Electron Microscopy Sciences (1 mentions)
2 protocols using rhodamine red
1
Visualizing Coronary Arteriole Proteins
2
Localization of Vascular ETA Receptors
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