Ab91413

Manufactured by Abcam

Sourced in United Kingdom, China

Ab91413 is a rabbit monoclonal antibody that recognizes the human EGFR protein. This antibody is intended for use in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ab91413

Western Blot Analysis of CD36 and NLRP3

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were extracted using a bicinchoninic acid protein kit (Beyotime, China) after lysing cells in RIPA buffer (Beyotime, China). Extracted proteins were separated by 10% SDS-PAGE and transferred to polyvinylidene membranes (Millipore, USA). Membranes were washed with Tris-Buffered Saline Tween (TBST), blocked with 5% non-fat milk for 1 h, and then incubated with a rabbit monoclonal anti-CD36 antibody (1: 1000; ab133625, Abcam, UK) or a rabbit monoclonal anti-NLRP3 antibody (1: 500; ab91413, Abcam, UK) overnight at 4°C. After being washed with TBST, membranes were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1: 20 000; ZSGB, China) for 1 h at room temperature. Finally, signals were detected with an enhanced chemiluminescence kit (Millipore, USA).

Yang X., Wu Y., Li Q., Zhang G., Wang M., Yang H, & Li Q. (2018). CD36 Promotes Podocyte Apoptosis by Activating the Pyrin Domain-Containing-3 (NLRP3) Inflammasome in Primary Nephrotic Syndrome. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 6832-6839.

+ Open protocol

+ Expand

Cytotoxicity Assay Protocol for Cell Stress Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Bilirubin, ethidium bromide (EtBr), Hoechst 33342, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), human serum albumin (HSA), and trypan blue were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). Ethidium homodimer-2 (EthD2) was purchased from Invitrogen (Thermo Fisher Scientific). VX-765 was purchased from Selleck Chemicals (Houston, TX, USA). Anti-caspase-1 antibody (ab1872) and anti-NLR family pyrin domain containing 3 (NLRP3) (ab91413) were purchased from Abcam (Cambridge, UK). Anti-glial fibrillary acidic protein (GFAP) antibody (3670), anti-β-actin (3700), an HRP-conjugated anti-rabbit secondary antibody (7074P2), and an HRP-conjugated anti-mouse secondary antibody (7076P2) were purchased from Cell Signaling Technology. IL-1β enzyme-linked immunosorbent assay (ELISA) kit and IL-18 ELISA kit were from USCN Life Science, Inc. (Wuhan, China). Lactate dehydrogenase (LDH) cytotoxicity detection kit was obtained from Beyotime Biotechnology (Shanghai, China). All other chemicals and reagents were from Beyotime Biotechnology unless otherwise specified.

Feng J., Li M., Wei Q., Li S., Song S, & Hua Z. (2018). Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes. Journal of Neuroinflammation, 15, 23.

+ Open protocol

+ Expand

Western Blot Analysis of NLRP3 Inflammasome

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples were collected and prepared. After electrophoresis and membrane transfer, the sample was incubated with primary antibodies. The primary antibodies were NLRP3 (1:1000; #ab91413; Abcam), caspase‐1 (1:500; #sc‐56036; Santa), ASC (1:1000; #sc‐514414; Santa), cathepsin B (1:1000; CST, #31718), β‐actin(1:1000; #BM0627; BOSTER, China), and β‐tubulin (1:1000; #A05397‐1; BOSTER). And then, membranes were treated with anti‐rabbit IgG (1:1500; #5127; CST) or anti‐mouse IgG (1:1500; #93702; CST). All protein bands were quantitated by Image J software (NIH, Bethesda, USA).

Lian D., Zhu L., Yu Y., Zhang X., Lin Y., Liu J., Han R., Guo Y., Cai D., Xiao W., Chen Y., He H., Xu D., Zheng C., Wang X., Huang Y, & Chen Y. (2022). Kakonein restores hyperglycemia‐induced macrophage digestion dysfunction through regulation of cathepsin B‐dependent NLRP3 inflammasome activation. Journal of Leukocyte Biology, 112(1), 143-155.

+ Open protocol

+ Expand

Inflammasome Pathway Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-IL-1β antibody (ab106015), anti-caspase-4 antibody (ab25898), anti-caspase-5 antibody (ab40887), anti-GSDMD antibody -C-terminal (ab228824), and anti-Urotensin II antibody-C-terminal (ab194676) were purchased from Abcam (UK, London, Abcam) used for western blot, IF and IHC methods. Anti-caspase-1 antibody (D7F10) was obtained from Cell Signaling Technology (US, Trask Lane Danvers, CST) and was used in western blot, IF, and IHC. Anti-NLRP -3 antibody (Beijing, China, Wanleibio) was used in IHC and IF, and anti-NLRP-3 antibody (ab91413) was obtained from Abcam for western blot.

Pan Y.J., Zhang M.Z., He L.H., Feng J, & Zhang A.H. (2021). Expression of urotensin II is positively correlated with pyroptosis-related molecules in patients with severe preeclampsia. Clinical and experimental hypertension (New York, N.Y. : 1993), 43(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!