Sma cy3 clone 1a4

Manufactured by Merck Group

Sourced in United States

SMA)-Cy3 (clone 1A4) is a laboratory reagent used for the detection and visualization of specific target proteins or molecules. It is a conjugate of the Sma1 monoclonal antibody and the Cy3 fluorescent dye. This product is designed for use in various research applications that require the specific targeting and labeling of the target of interest.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti gs, by Merck Group (1 mentions) Goat anti lyve 1, by R&D Systems (1 mentions) Rabbit anti ck19, by Proteintech (1 mentions) Hoechst 33342, by Cell Signaling Technology (1 mentions) Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Bz x710, by Keyence (1 mentions)

Close spelling variants of this product

Clone 1A4 (24 citations)

2 protocols using sma cy3 clone 1a4

Immunofluorescence Staining of Lymphatic Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: goat anti-LYVE-1 (polyclonal, R&D, AF2089), rat anti-PECAM-1 (clone 5D2.6 and IG5.1, provided by D. Vestweber, Max Planck Institute for Molecular Biomedicine Munster), rabbit anti-LYVE-1 (polyclonal, Reliatech), goat anti-LDLR (polyclonal, R&D, AF2255), rabbit anti-GS (polyclonal, Sigma-Aldrich, G2781), rabbit anti-CK19 (polyclonal, proteintech, 14965-1-AP), mouse anti-smooth muscle actin (SMA)-Cy3 (clone 1A4, Sigma), rat anti-Lyve-AlexaFluor594 (clone 223322, R&D), secondary antibodies coupled to AlexaFluor488, 568 and 647 (Invitrogen).

Bobe S., Beckmann D., Klump D.M., Dierkes C., Kirschnick N., Redder E., Bauer N., Schäfers M., Erapaneedi R., Risse B., van de Pavert S.A, & Kiefer F. (2022). Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice. Frontiers in Cell and Developmental Biology, 10, 949896.

+ Open protocol

+ Expand

Immunocytochemistry of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunocytochemistry of culture cells was performed as described previously (Shirakihara et al, 2007), using monoclonal antibodies to Tie2 (bs‐1300R; Bioss Antibodies, Woburn, MA, USA) and SMA‐Cy3 (clone 1A4; SIGMA). Samples were incubated with secondary antibodies: Alexa Fluor 488 Donkey Anti‐Rabbit IgG (H+L; A‐21206; Thermo Fisher Scientific). Stained cells were photographed under a fluorescence microscope (BZ‐X710; KEYENCE, Osaka, Japan) with 20× objective lenses. Nuclei were counterstained with Hoechst 33342 (4082; Cell Signaling Technology).

Akatsu Y., Takahashi N., Yoshimatsu Y., Kimuro S., Muramatsu T., Katsura A., Maishi N., Suzuki H.I., Inazawa J., Hida K., Miyazono K, & Watabe T. (2019). Fibroblast growth factor signals regulate transforming growth factor‐β‐induced endothelial‐to‐myofibroblast transition of tumor endothelial cells via Elk1. Molecular Oncology, 13(8), 1706-1724.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!