Log In Sign up
The largest database of trusted experimental protocols

Alexa fluor 488 conjugated mab against phosphorylated

Manufactured by BD
Visit Supplier

Alexa Fluor 488-conjugated mAb against phosphorylated is a fluorescently labeled monoclonal antibody that binds to phosphorylated proteins. It can be used in various immunoassays and imaging applications to detect and quantify phosphorylation events.

Automatically generated - may contain errors

Lab products found in correlation

Cytofix and phosflow perm wash buffers, by BD (2 mentions) Goat f ab 2 anti human iga g m, by Jackson ImmunoResearch (2 mentions) Alexa fluor 647 conjugated mab against phosphorylated, by BD (2 mentions) Aurora cytometer, by FlowJo (2 mentions)

2 protocols using alexa fluor 488 conjugated mab against phosphorylated

1

Phosphorylation of Syk and PLC-γ2 in BCR-stimulated PBMCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs stained with mAbs against CD19, CD20, CD3, CD27, CD21, IgD, and BV421-conjugated RBD-B.1 were resuspended in RPMI 1640–10% FBS and stimulated with anti-BCR as described previously (Kardava et al., 2018 (link)), with the following modifications. The cells were stimulated at 37°C for 2 min with 10 μg/ml goat F(ab’)2 anti-human IgA/G/M (# 109-006-064 from Jackson ImmunoResearch Laboratories). For the detection of phosphorylated signalling intermediates, cells were fixed and permeabilized using BD Cytofix and Phosflow Perm/Wash buffers (BD Biosciences) and stained with Alexa Fluor 488-conjugated mAb against phosphorylated Syk (p-Y348) and Alexa Fluor 647-conjugated mAb against phosphorylated PLC-2 (p-Y759) (BD Biosciences). The samples were acquired on an Aurora cytometer and analysis was performed by FlowJo V10.
Buckner C.M., Kardava L., Merhebi O.E., Narpala S.R., Serebryannyy L., Lin B.C., Wang W., Zhang X., de Assis F.L., Kelly S.E., Teng I.T., McCormack G.E., Praiss L.H., Seamon C.A., Rai M.A., Kalish H., Kwong P.D., Proschan M.A., McDermott A.B., Fauci A.S., Chun T.W, & Moir S. (2022). Recent SARS-CoV-2 infection abrogates antibody and B-cell responses to booster vaccination. medRxiv.
+ Open protocol
+ Expand
2

BCR Stimulation and Signaling Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs stained with mAbs against CD19, CD20, CD3, CD27, CD21, IgD, and BV421-conjugated RBD-B.1 were resuspended in RPMI 1640–10% FBS and stimulated with anti-BCR as described previously (Kardava et al., 2018 (link)), with the following modifications. The cells were stimulated at 37°C for 2 min with 10 μg/ml goat F(ab’)2 anti-human IgA/G/M (# 109-006-064 from Jackson ImmunoResearch Laboratories). For the detection of phosphorylated signaling intermediates, cells were fixed and permeabilized using BD Cytofix and Phosflow Perm/Wash buffers (BD Biosciences) and stained with Alexa Fluor 488-conjugated mAb against phosphorylated Syk (p-Y348) and Alexa Fluor 647-conjugated mAb against phosphorylated PLC-2 (p-Y759) (BD Biosciences). The samples were acquired on an Aurora cytometer and analysis was performed by FlowJo V10.
Buckner C.M., Kardava L., El Merhebi O., Narpala S.R., Serebryannyy L., Lin B.C., Wang W., Zhang X., Lopes de Assis F., Kelly S.E., Teng I.T., McCormack G.E., Praiss L.H., Seamon C.A., Rai M.A., Kalish H., Kwong P.D., Proschan M.A., McDermott A.B., Fauci A.S., Chun T.W, & Moir S. (2022). Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses. Cell, 185(23), 4333-4346.e14.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!