Log In Sign up
The largest database of trusted experimental protocols

Livedead fixable

Manufactured by Thermo Fisher Scientific
Visit Supplier

LiveDead Fixable is a fluorescent dye-based reagent used for the viability analysis of cells in flow cytometry applications. It enables the discrimination between live and dead cells by staining dead cells with a fluorescent dye.

Automatically generated - may contain errors

Lab products found in correlation

Ab8295, by Abcam (2 mentions) A11001, by Thermo Fisher Scientific (2 mentions) Bx41 fluorescence microscope, by Olympus (2 mentions)

Spelling variants of this product

AquaAmine LiveDead Fixable viability stain (3 citations) LiVEDEAD® Fixable Aqua Dead Cell Stain Kits (2 citations)

2 protocols using livedead fixable

1

Cardiomyocyte Nucleation Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hearts were digested with 1mg/mL collagenase type II via Langendorff retroaortic perfusion. After digestion, atria and valves were removed and ventricular tissue alone was triturated in Kruftbrühe (KB) solution (70mM potassium aspartate, 40mM KCl, 15mM KH2PO4, 10mM glucose, 10mM taurine, 0.5mM EGTA, 10mM sodium pyruvate, 10mM HEPES, 5mM BDM, 0.5% BSA), filtered through a 250-μm nylon mesh, stained with LiveDead Fixable (ThermoFisher, L10120) for 20 min at room temperature and then fixed in 2% PFA at room temperature for 15 min. Fixed ventricular cell suspensions were stained for cTnT (1:1,000, Abcam ab8295) overnight at 4°C followed by goat anti-mouse secondary (1:500, ThermoFisher A11001) and DAPI. Cell suspensions were then pipetted across a slide and coverslipped. Cardiomyocyte nucleation was quantified on an Olympus BX41 fluorescence microscope with a 20x objective. Only live cardiomyocytes were counted for mono-, bi-, tri- and tetranucleation; at least 600 cells were counted per heart. An unpaired, two-tailed Student t-test was used to assess statistical significance when only two groups were compared.
Gan P., Patterson M., Velasquez A., Wang K., Tian D., Windle J.J., Tao G., Judge D.P., Makita T., Park T.J, & Sucov H.M. (2019). Tnni3k alleles influence ventricular mononuclear diploid cardiomyocyte frequency. PLoS Genetics, 15(10), e1008354.
+ Open protocol
+ Expand
2

Quantification of Cardiomyocyte Nucleation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hearts were digested with 1 mg/mL collagenase type II via Langendorff retroaortic perfusion15 (link). After digestion, atria and valves were removed and ventricular tissue alone was triturated in Kruftbrühe (KB) solution15 (link), filtered through a 250-μm mesh, stained with LiveDead Fixable (ThermoFisher, L10120) for 20 min at room temperature and then fixed in 2% PFA at room temperature for 15 min. Fixed ventricular cell suspensions were stained for cTnT (1:1,000, Abcam ab8295) overnight at 4 °C followed by goat anti-mouse secondary (1:500, ThermoFisher A11001) and DAPI. Cell suspensions were then pipetted across a slide and coverslipped. Cardiomyocyte nucleation was quantified on an Olympus BX41 fluorescence microscope with a 20× objective. Only live car-diomyocytes were counted for mono-, bi-, tri- and tetranucleation; at least 200 cells were counted per heart. An unpaired, two-tailed Student t-test was used to assess statistical significance when only two groups were being compared. One-way ANOVA with Bonferroni correction was used to assess statistical significance across >2 groups.
Patterson M., Barske L., Van Handel B., Rau C.D., Gan P., Sharma A., Parikh S., Denholtz M., Huang Y., Yamaguchi Y., Shen H., Allayee H., Crump J.G., Force T.I., Lien C.L., Makita T., Lusis A.J., Kumar S.R, & Sucov H.M. (2017). Frequency of mononuclear diploid cardiomyocytes underlies natural variation in heart regeneration. Nature genetics, 49(9), 1346-1353.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!