The bile salt hydrolase (BSH)-positive strain Lactobacillus acidophilus DRU, the hydrogen peroxide (H2O2) producer Lactobacillus acidophilus ATCC 4356, and the reference strain Lactobacillus rhamnosus GG (ATCC 53103) were routinely cultured in de Man Rogosa and Sharpe (MRS, Biolife, Italy) medium plus 100 mg/L of cycloheximide (Merck, Germany) at 37 °C under anaerobic conditions, using Anaerocult C (Merck, Milan, Italy). The haemolytic positive strains Streptococcus pyogenes ATCC 19615 and Streptococcus pneumoniae ATCC 6303 were cultured on Brain-Heart Infusion (BHI, Becton Dickinson GmbH, Germany) at 37 °C under 5% CO2 conditions. Escherichia coli 555, E. coli ATCC 25922, E. coli ATCC 700414, and Staphylococcus aureus ATCC 6538 were routinely cultured on Trypticase Soy Broth medium (Oxoid, Milan) at 37 °C, under aerobic conditions. Listeria monocytogenes DSM 12464 strain was reactivated in BHI broth at 30 °C. Gardnerella vaginalis ATCC 14018 was cultured on Casman’s medium base added of 5% of rabbit blood (VWR, Milan, Italy) at 37 °C. Candida albicans ATCC 10231, Candida krusei ATCC 14243, Candida glabrata ATCC 90030, Candida parapsilosis ATCC 90018, Candida lusitaniae ATCC 200951 and Candida tropicalis ATCC 13803 were cultured on Yeast Mold Broth (Conda, Madrid, Spain) at 28 °C in aerobic conditions.
Yeast mold broth
Manufactured by Condalab
Sourced in Spain
Yeast Mold Broth is a culture medium designed to support the growth of yeasts and molds. It provides essential nutrients and a suitable pH environment for the cultivation of these microorganisms. The broth's composition and properties facilitate the isolation, identification, and enumeration of yeasts and molds in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Mrs broth, by Condalab (2 mentions)
Centrofriger bl 2, by J.P. Selecta (2 mentions)
Sterile quarter strength ringer s solution, by Thermo Fisher Scientific (2 mentions)
Brain heart infusion bhi, by BD (1 mentions)
Anaerocult c, by Merck Group (1 mentions)
Trypticase soy broth medium, by Thermo Fisher Scientific (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
3 protocols using yeast mold broth
1
Cultivation of Diverse Microbial Strains
2
Isolation and Cultivation of Probiotic Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lactobacillus plantarum MNC 21 and Saccharomyces cerevisiae MNC21Y isolated from Obushera were used in the study. Lb. plantarum MNC21 was grown in 100 ml of MRS broth (CONDA, Madrid, Spain) at 30°C for 24 h. S. cerevisiae MNC21Y was grown in 100 ml of Yeast Mold Broth (CONDA, Madrid, Spain) at 30°C for 24 h. The cells were separately centrifuged at 7500 x g for 10 min (Centrofriger -BL II, JP Selecta, Barcelona, Spain) and the pellets washed thrice using sterile quarter strength Ringer's solution (Oxoid Limited, Basingstroke, Hampshire, England). The pellets were then separately suspended in 10 ml of sterile quarter strength Ringer's solution.
3
Isolation and Preparation of Probiotic Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lactobacillus plantarum MNC 21 and Saccharomyces cerevisiae MNC21Y isolated from Obushera [2] (link) were used in the study. Lb. plantarum MNC21 was grown in 100 ml of MRS broth (CONDA, Madrid, Spain) at 30°C for 24 h. S. cerevisiae MNC21Y was grown in 100 ml of Yeast Mold Broth (CONDA, Madrid, Spain) at 30°C for 24 h. The cells were separately centrifuged at 7500 x g for 10 min (Centrofriger -BL II, JP Selecta, Barcelona, Spain) and the pellets washed thrice using sterile quarter strength Ringer's solution (Oxoid Limited, Basingstroke, Hampshire, England). The pellets were then separately suspended in 10 ml of sterile quarter strength Ringer's solution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!