The tested pure PCS constituents (≥95%) of PS and PSD and the standard selective MAO-AI clorgyline (CLORG) were obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Hank's Balanced Salt Solution (HBSS), HEPES, benzylamine HCl, IPS, 6-PN, NBI, BIO-A, the selective MAO-BI standard selegiline (Deprenyl®) (DEP), and other chemicals and chromatography supplies were purchased from Sigma-Aldrich (St Louis, MO, USA). Human MAO-A and MAO-B, derived from a recombinant baculovirus infected insect cells, and measured in active units (U) were purchased from Sigma-Aldrich and received in a buffer solution at pH 7.4. The China originated PCS were obtained from East Earth Trade Winds (Redding, CA, USA), and the MAO-Glo™ kits from Promega (Madison, WI, USA).
Human mao a and mao b
Manufactured by Merck Group
Human MAO-A and MAO-B are recombinant enzymes produced in a bacterial expression system. MAO-A and MAO-B are isoforms of the monoamine oxidase enzyme, which catalyze the oxidative deamination of monoamine neurotransmitters. These recombinant enzymes can be used for in vitro studies and assays related to monoamine oxidase activity.
Automatically generated - may contain errors
Lab products found in correlation
Hank s balanced salt solution hbss, by Merck Group (1 mentions)
Bio a, by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Kynuramine dihydrobromide, by Merck Group (1 mentions)
Kavain, by Merck Group (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
2 protocols using human mao a and mao b
1
MAO-A and MAO-B Inhibition Assay
2
Fluorescence Spectrophotometry of Recombinant MAO
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Fluorescence spectrophotometry was carried out using a Varian Cary Eclipse fluorescence spectrophotometer. Microsomes from insect cells containing recombinant human MAO-A and MAO-B (5 mg protein/mL) and kynuramine dihydrobromide were obtained from Sigma. (±)-Kavain (> 95 %), (+)-7,8-dihydrokavain (> 98 %), (+)-methysticin (> 98 %), (+)-7,8-dihydromethysticin (> 90 %), yangonin (> 95 %), desmethoxyyangonin (> 95 %), and curcumin (> 98 %) were purchased from Sigma. (+)-Kavain (98 %) was obtained from Carbosynth.
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