Deuterated L-citrulline (L-citrulline-d6) was obtained from Sapphire Bioscience (Sapphire Bioscience, Redfern, Australia). High purity water was obtained using a MilliQ Synergy UV Ultrapure water system (Merck Millipore, Sydney, Australia). Acetonitrile (Liquid Chromatography-Mass Spectrometry, LC-MS, Grade), 2-propanol and formic acid (High Performance Liquid Chromatography, HPLC, Grade) were obtained from Merck Millipore (Merck Millipore, Melbourne, Australia). All other laboratory grade chemicals and reagents were purchased from Sigma-Aldrich (Sigma-Aldrich, Sydney, Australia). DDAH1 over-expression was performed as reported by Tommasi et al.18 (link) A single batch of lysate prepared from DDAH1-expressing HEK293Tcells was used for all in vitro experiments.
Milliq synergy uv ultrapure water system
Manufactured by Merck Group
Sourced in Australia, Germany
The MilliQ Synergy UV Ultrapure water system is a laboratory equipment designed to produce ultrapure water. It utilizes a multi-stage purification process to remove impurities and deliver water of the highest quality. The system includes features such as UV treatment and resistivity monitoring to ensure consistent water purity.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Ketoconazole, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Gefitinib, by LC Laboratories (1 mentions)
Lapatinib, by LC Laboratories (1 mentions)
Sorafenib, by LC Laboratories (1 mentions)
2 protocols using milliq synergy uv ultrapure water system
1
Deuterated L-citrulline quantification protocol
2
Hepatic Metabolism of Anticancer Drugs
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Gefitinib, lapatinib and sorafenib were purchased from LC Laboratories (Woburn, MA, USA). Glucose−6−phosphate (G−6−P), G−6−P dehydrogenase (G−6−PD), nicotinamide adenine dinucleotide phosphate (NADP), testosterone and ketoconazole were purchased from Sigma−Aldrich (St. Louis, MO, USA). Acetonitrile, ammonium acetate, acetic acid, dimethyl sulfoxide (DMSO) and methanol were purchased from Merck KGaA (Darmstadt, Germany). High purity water was from MilliQ Synergy UV Ultrapure water system (Merck KGaA, Darmstadt, Germany). All other solvents and reagents were analytical grade or higher. Pooled human liver microsomes (HLMs; from 50 donors) were purchased from Gibco BRL Life Technologies (Grand Island, NY, USA).
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