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Renilla luciferase control plasmid

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The Renilla-luciferase control plasmid is a laboratory tool used to express the Renilla luciferase protein, which can be used as a reporter gene in various experimental setups. The plasmid contains the necessary genetic elements for the expression of the Renilla luciferase enzyme in host cells.

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Lab products found in correlation

Mirna mimic, by Thermo Fisher Scientific (2 mentions) Glomax 96 luminometer, by Promega (2 mentions)

2 protocols using renilla luciferase control plasmid

1

Luciferase Assay for miRNA Targeting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wildtype and mutant pMIR-LCOR-3′UTR repoters were transfected into HeLa cells together with the Renilla-luciferase control plasmid (Ambion). 200ng of reporter plasmid was co-transfected with the Renilla-luciferase control plasmid and 10pM of miRNA mimics (Applied Biosystems; Life Technologies). Lipofectamine 2000 was used as the transfection reagent. Cells were lysed 24 hours after transfection and analyzed for luciferase activity using the Glomax 96 Luminometer (Promega).
Celià-Terrassa T., Liu D., Choudhury A., Hang X., Wei Y., Zamalloa J., Alfaro-Aco R., Chakrabarti R., Jiang Y.Z., Koh B.I., Smith H., DeCoste C., Li J.J., Shao Z.M, & Kang Y. (2017). Normal and cancer mammary stem cells evade interferon-induced constraint through the miR-199a-LCOR Axis. Nature cell biology, 19(6), 711-723.
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2

Luciferase Assay for miRNA Targeting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wildtype and mutant pMIR-LCOR-3′UTR repoters were transfected into HeLa cells together with the Renilla-luciferase control plasmid (Ambion). 200ng of reporter plasmid was co-transfected with the Renilla-luciferase control plasmid and 10pM of miRNA mimics (Applied Biosystems; Life Technologies). Lipofectamine 2000 was used as the transfection reagent. Cells were lysed 24 hours after transfection and analyzed for luciferase activity using the Glomax 96 Luminometer (Promega).
Celià-Terrassa T., Liu D., Choudhury A., Hang X., Wei Y., Zamalloa J., Alfaro-Aco R., Chakrabarti R., Jiang Y.Z., Koh B.I., Smith H., DeCoste C., Li J.J., Shao Z.M, & Kang Y. (2017). Normal and cancer mammary stem cells evade interferon-induced constraint through the miR-199a-LCOR Axis. Nature cell biology, 19(6), 711-723.
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