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Rat urinary protein assay kit

Manufactured by Chondrex
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The Rat Urinary Protein Assay Kit is a laboratory tool designed to quantify protein levels in rat urine samples. This kit utilizes a colorimetric method to determine the concentration of proteins present in the samples.

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Lab products found in correlation

Rat elisa kit, by Merck Group (1 mentions) Lbis rat albumin elisa kit, by Fujifilm (1 mentions) Labassay creatinine kit, by Fujifilm (1 mentions) Ab223858, by Abcam (1 mentions) Rat albumin elisa kit, by Abcam (1 mentions)

4 protocols using rat urinary protein assay kit

1

Biomarker Measurement in Rat Model

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Hemoglobin was measured using an automated meter (Germaine Labs AimStrip Hemoglobin Test System, catalog# 23‐111‐280, Fisher Scientific). Plasma samples were analyzed for blood urea nitrogen (BUN) and creatinine using colorimetric kits from BioAssay Systems (Hayward CA). Urine samples were diluted 30x for creatinine (BioAssay Systems) and 5x for protein measurements (Rat Urinary Protein Assay Kit, Chondrex, Inc., Redmond WA). Creatinine clearance normalized to body weight (CrCl, mL/min*kg) was calculated from 24 hours urine collections using the formula: [urine Cr x urine volume]/[serum Cr x 1440 x body weight]. Plasma C‐reactive protein (CRP) and galectin‐3 were measured using rat ELISA kits from Sigma‐Aldrich (St. Louis MO) and MyBioSource (San Diego CA).
Lau W.L., Khazaeli M., Savoj J., Manekia K., Bangash M., Thakurta R.G., Dang A., Vaziri N.D, & Singh B. (2018). Dietary tetrahydrocurcumin reduces renal fibrosis and cardiac hypertrophy in 5/6 nephrectomized rats. Pharmacology Research & Perspectives, 6(2), e00385.
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2

Quantitative Urinary Biomarker Analysis

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Total urine samples were collected over 24 h from each animal in the metabolic cages at each time-point. Urine samples were used immediately or stored at -20˚C until required. Urinary protein, albumin and creatinine levels were measured using a Rat Urinary Protein assay kit (Chondrex, Inc.), a LBIS™ Rat Albumin ELISA kit (FUJIFILM Wako Pure Chemical Corporation) and a LabAssay™ Creatinine kit (FUJIFILM Wako Pure Chemical Corporation) respectively, according to the manufacturer's protocol.
Fujitaka K., Murakami T., Takeuchi M., Kakimoto T., Mochida H, & Arakawa K. (2020). mRNAs in urinary nano-extracellular vesicles as potential biomarkers for non-invasive kidney biopsy. Biomedical Reports, 14(1), 11.
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3

Biomarkers for Kidney Function Assessment

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We measured hemoglobin by using an automated meter (Germaine Lab AimStrip Hemoglobin Test System, catalog# 23–111‐280, Fisher Scientific). Blood urea nitrogen (BUN) and creatinine (Cr) were analyzed with colorimetric kits from BioAssay Systems, with 30x dilution used for urine creatinine. Urine albumin was measured with 1000x dilution using the Rat Albumin ELISA kit (ab108789, Abcam) and urine protein from 24‐hour urine collection was measured using the Rat Urinary Protein Assay Kit (Chondrex Inc.). Creatinine clearance (mL/min*kg) was calculated as follows: [urine Cr × urine volume]/[serum Cr × 1440 × body weight]. Proteinuria per 24 h period was normalized to body weight and to creatinine clearance. Plasma levels of kidney injury molecule‐1 (KIM‐1) were measured with 4x dilution using a rat ELISA kit (ab223858, Abcam). Plasma fructosamine was measured via spectrophotometry at Phoenix Lab.
Khazaeli M., Nunes A.C., Zhao Y., Khazaeli M., Prudente J., Vaziri N.D., Singh B, & Lau W.L. (2023). Tetrahydrocurcumin Add‐On therapy to losartan in a rat model of diabetic nephropathy decreases blood pressure and markers of kidney injury. Pharmacology Research & Perspectives, 11(2), e01079.
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4

Comprehensive Metabolic Panel Analysis

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The plasma glucose concentration was determined using an enzymatic colorimetric method using a commercial kit (Sigma Aldrich, San Luis, MO, USA). Total urinary protein levels were measured using a Rat Urinary Protein Assay Kit (Chondrex, Redmond, WA, USA). In serum, creatinine was evaluated using a QuantiChrom™ Protein Creatinine Ratio Assay Kit (BioAssay Systems, San Francisco, CA, USA), urea, uric acid and blood urea nitrogen test (BUN) were measured using Assay Kits (Abcam, Cambridge MA, USA) according to the manufacturer’s instructions. Kidneys were dissected and weighed (wet weight).
Pérez Gutierrez R.M., García Campoy A.H., Paredes Carrera S.P., Muñiz Ramirez A., Mota Flores J.M, & Flores Valle S.O. (2019). 3′-O-β-d-glucopyranosyl-α,4,2′,4′,6′-pentahydroxy-dihydrochalcone, from Bark of Eysenhardtia polystachya Prevents Diabetic Nephropathy via Inhibiting Protein Glycation in STZ-Nicotinamide Induced Diabetic Mice. Molecules, 24(7), 1214.
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