The treatment of clinical specimens was as previously described [15 (link)]. The total RNA was extracted using the pre-installed viral total NA kit-Flex (Fisher Scientific, Labserv, Cat. no. KFRPF-805296). SARS-CoV-2 RNA was analyzed with the commercial rRT-PCR kit (DaAn Gene, Guangzhou, China. Cat. no. DA0931) targeting the ORF1ab and nucleocapsid (N) genes. Amplification was performed on the Applied Biosystems™ 7500 real-time fluorescent PCR instrument (ThermoFisher Scientific, Waltham, MA, USA). A CT value <40 was considered as a SARS-CoV-2 positive sample.
Applied biosystems 7500 real time fluorescent pcr instrument
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Applied Biosystems™ 7500 real-time fluorescent PCR instrument is a thermal cycler designed for quantitative real-time PCR (qPCR) analysis. It is capable of detecting and quantifying nucleic acid targets in real-time using fluorescent dyes or probes.
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Lab products found in correlation
2 protocols using applied biosystems 7500 real time fluorescent pcr instrument
1
SARS-CoV-2 RNA Detection via rRT-PCR
2
SARS-CoV-2 Detection by rRT-PCR
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Throat swab specimens were treated as previously described.32 (link) Total RNA was extracted using a virus nucleic acid preload kit (Magen Biotechnology Co., Ltd., Guangzhou, China). SARS-CoV-2 RNA was analyzed with a commercial rRT-PCR kit (DaAn Gene, Guangzhou, China) targeting the ORF1ab and nucleocapsid (N) genes. Amplification was performed on an Applied Biosystems™ 7500 real-time fluorescent PCR instrument (Thermo Fisher Scientific, Waltham, MA, USA). A CT value < 40 was considered SARS-CoV-2 RNA positive in throat swab samples.
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