Siinfekl h 2kb

d-Mannose, allyl alcohol, N-acryloxysuccinimide (NAS), ammonium persulfate (APS), N-(3-aminopropyl) methacrylamide hydrochloride (Apm), ethylene glycol dimethacrylate (EMA), and fluorescamine were purchased from Aladdin (Shanghai, China). Ovalbumin (OVA), and N,N,N′,Nʹ′-tetramethylethylenediamine (TEMED) were purchased from Sigma‒Aldrich (St. Louis, MO, USA). Zoledronic acid (Zol) was purchased from J&K Scientific Co., Ltd. (Beijing, China). Roswell Park Memorial Institute-1640 (RPMI-1640) medium and fetal bovine serum (FBS) were purchased from Gibco (Beijing, China). Granulocyte-macrophage colony stimulating factor (GM-CSF) was purchased from Bio-Techne (St. Louis, MO, USA). Fluorochrome-labeled anti-mouse CD11c, SIINFEKL/H-2Kb, CD40, CD80, CD86, F4/80, CD4, CD8a, IL-4, IFN-γ, CD44 and CD62L antibodies were purchased from eBioscience (San Diego, CA, USA). BCA protein assay kit and enzyme-linked immune sorbent assay (ELISA) kits for IL-6, TNF-α, IFN-γ were purchased from Thermo Fisher Scientific (San Diego, CA, USA). Mouse IFN-γ ELISPOT kit was purchased from Dakewe Biotech (Beijing, China). Ninety-six well plates were obtained from NEST Biotechnology (Wuxi, China). All the other chemicals used are analytical grade or HPLC grade, and ultrapure water was used for solution preparation.

Formalin‐fixed paraffin liver sections were stained with haematoxylin and eosin to visualize biliary inflammation. For evaluation of the liver‐infiltrating congenic OT‐1 cells (CD45.1+) and their interaction with cholangiocytes (CK19+), cryosections of liver tissue were stained with PE‐labelled antibody to CD45.1 (BioLegend) and primary antibody to CK19 (DSHB Hybridoma Product TROMA‐III) together with AF488‐labelled secondary antibody. Nuclei were stained with Hoechst 33342. To visualize MHC I‐restricted SIINFEKL presentation by cholangiocytes or cross‐presentation by LSECs, SIINFEKL‐H2K^b (eBioscience) was costained with CK19 antibody (DSHB Hybridoma Product TROMA‐III) or LYVE‐1 antibody (R&D systems). As indicated in the figure legends, AF488‐ or AF546‐labelled secondary antibodies have been used (Invitrogen).