63 1.4 plan apochromat

Manufactured by Zeiss

The ×63/1.4 Plan Apochromat is a high-performance objective lens designed for microscopy applications. It provides a numerical aperture of 1.4 and a magnification of 63×, delivering exceptional optical performance and resolution. This lens is optimized to minimize chromatic and spherical aberrations, ensuring accurate and detailed image reproduction.

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Lab products found in correlation

Lsm 880, by Zeiss (2 mentions) 63 1.4 plan apochromat dic, by Zeiss (1 mentions) Lsm 700 microscope, by Zeiss (1 mentions) Zen 2, by Zeiss (1 mentions) Ec plan neofluar, by Zeiss (1 mentions) Axiocam 105, by Zeiss (1 mentions) Axio imager 2, by Zeiss (1 mentions)

Close spelling variants of this product

Plan-Apochromat 63×/1.4 oil DIC objective lens Plan-Apochromat 63×/1.4 oil Plan Apochromat 63×/1.4 NA oil immersion lens Plan-Apochromat 63×/1.4 oil lens Plan-Apochromat 63×/1.4 NA DIC M27 Oil objective ×63/1.4 Plan Apochromat DIC Plan-Apochromat 63×/1.4 Oil DIC M27 objective lens Plan-Apochromat 63×/1.4 oil immersion objective lens Plan-apochromat X 63 (NA 1.4) oil immersion objective Plan-Apochromat 63×/1.4 numerical aperture oil objective

2 protocols using 63 1.4 plan apochromat

Quantitative Analysis of pMLC2 Localization

Cited 1 time

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Images from fixed tissues were acquired using an LSM 700 microscope (Zeiss) equipped with a ×63/1.4 Plan Apochromat DIC oil immersion objective. Quantifications of pMLC2 fluorescence intensities at the back, the front, and the side of the cluster were performed as previously described^{6 (link)}.
For more precise analysis of pMLC2 localization and for the line scan analysis, we acquired images from fixed tissues with the Airyscan detector of a Zeiss LSM880 confocal equipped with a ×63/1.4 Plan Apochromat oil immersion objective. Line scans were performed on the Zen Blue software and plotted in GraphPad Prism. Specific line scan were chosen according to the base of the protrusion. The protrusion bases were determined manually by its neck shape (opposite negative curvature shapes). The signal were normalized to the maximal signal of pSqh intensity.

Plutoni C., Keil S., Zeledon C., Delsin L.E., Decelle B., Roux P.P., Carréno S, & Emery G. (2019). Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration. Nature Communications, 10, 3940.

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Embryonic Cartilage Development Imaging

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E17.5 embryos were decalcified in 20% EDTA at 4°C for 3 days, while E12.5 and E13.5 embryos did not require decalcification. Embryos were fixed in 4% paraformaldehyde (PFA) for 48 hours and embedded in paraffin for sectioning in the sagittal plane. 7μm sections were stained with 1% Safranin-O, 0.05% Fast Green, and 1% Hematoxylin, and then visualized by light microscopy (Axio Imager 2, Carl Zeiss) using 20×/0,5 EC Plan-Neofluar (Carl Zeiss) or 63×/1,4 Plan-Apochromat (Carl Zeiss) objectives. Imaging of sections was conducted with the Axiocam 105 color camera (Carl Zeiss) using Zen2™ software (Carl Zeiss). Morphological analysis was performed on eight E12.5, two E13.5, and ten E17.5 null and wild-type embryos. Aspect ratio was measured using ImageJ 1.52a (http://rsb.info.nih.gov/ij/), where the major axis was divided by the minor axis determined by Fit Ellipse measurements. Aspect ratio data were collected from five E17.5 embryos per genotype with three discs per embryo (15 discs).

Tessier S., Madhu V., Johnson Z.I., Shapiro I.M, & Risbud M.V. (2019). NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis. Developmental biology, 455(2), 369-381.

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