Multiple elisa kit

Isoproterenol hydrochloride, propranolol (Pro), and Griess reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Halt protease and phosphatase inhibitor cocktail were purchased from Pierce Biotechnology (Rockford, IL, USA). The antibody against HO-1 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); secondary anti-rabbit IgG (H and L, horseradish peroxidase-linked) was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH was purchased from BioLegend Inc. (San Diego, CA, USA) and the anti-SOD1 and SOD2, GPx, catalase, and Nrf2 antibodies were from Abcam (Cambridge, UK). A multiple ELISA kit was obtained from Merck Millipore (Darmstadt, Germany). Zoletil was obtained from Virbac Laboratories (Fort Worth, Texas, USA). Novolink™ Polymer Detection System was purchased from Leica (Newcastle, UK). All other chemicals were of analytical grade, including Avicel PH 102 (FMC Biopolymer, USA) and Ludiflash (Pharma Ingredients & Services, Bishop, Texas, USA).

The pro-inflammatory mediators TNF-α and IL-6 levels in serum were measured by using a multiple ELISA kit (Merck Millipore, Darmstadt, Germany) according to the manufacturer's instructions. The absorbance was measured by a microplate reader. The levels of mediators are expressed as pg/mg of protein.