Pathogreen histofluorescent stain

Manufactured by Biotium

Sourced in United States

PathoGreen is a histofluorescent stain developed by Biotium. It is designed to detect and visualize pathogenic organisms in biological samples.

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Lab products found in correlation

Novolink polymer detection system, by Leica (1 mentions) Anti ndufs4, by Novus Biologicals (1 mentions) Anti gfap, by Merck Group (1 mentions) Anti cd68, by Abcam (1 mentions) Cy3 goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Gapdh rabbit mab, by Cell Signaling Technology (1 mentions) Cy2 goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)

3 protocols using pathogreen histofluorescent stain

Immunohistochemical Analysis of Mitochondrial Proteins

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All chemicals were from Sigma. Antibodies used included the following: anti-NDUFS4 (1:100 for immunohistochemistry [IHC] and 1:1,000 for western blot [WB]) was from Novus Biologicals (catalog no. NBP1-31465); anti-CD68 (1:100) was from Abcam (catalog no. ab125212); anti-GFAP (1:1,000) was from Sigma-Aldrich (catalog no. G3893); anti-NDUFA9, and anti-SDHB (1:1,000) were from MitoSciences (catalog no. ab14713). Secondary antibodies were from Promega (catalog nos. W4011 [rabbit] and W4021 [mouse]). PathoGreen histofluorescent stain (1,000X in water) was from Biotium (catalog no. 80027-5mL). The Novolink Polymer Detection System was from Leica Biosystems (catalog no. RE7140-K). The anti-LY6A antibody (dilution 1:1,000) was from Abcam (catalog no. ab109211).

Silva-Pinheiro P., Cerutti R., Luna-Sanchez M., Zeviani M, & Viscomi C. (2020). A Single Intravenous Injection of AAV-PHP.B-hNDUFS4 Ameliorates the Phenotype of Ndufs4−/− Mice. Molecular Therapy. Methods & Clinical Development, 17, 1071-1078.

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Neuronal Degeneration Histofluorescent Staining

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PathoGreen™ Histofluorescent Stain (Biotium, CA, United States) was used to detect neuronal degeneration. Experiments were performed according to manufacturer’s instructions. Briefly, the brain was perfused and fixed with 4% PFA overnight at 4°C. Then, the brain was cut into 50 μm coronal slices, which were dried at 60°C for 30 min. Slices were incubated with 0.06% KMnO₄ and 1,000 × PathoGreen™ stock solution at RT for 10 min each. After air drying, slices were incubated with xylene and mounted onto glass slides. Images were captured using a laser scanning confocal microscope with a 40 × lens objective.

Morimoto K., Watanuki S., Eguchi R., Kitano T, & Otsuguro K.I. (2023). Short-term memory impairment following recovery from systemic inflammation induced by lipopolysaccharide in mice. Frontiers in Neuroscience, 17, 1273039.

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Immunofluorescence Analysis of Microglia and Astrocytes

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C1r mouse monoclonal antibody (mAb) (#sc-514105), goat anti-rabbit IgG-HRP (#sc-2004) and goat anti-mouse IgG-HRP (#sc-2005) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). CD11b/c mouse mAb (ab1211) was purchased from Abcam (Waltham, MA, USA). CD11b rabbit polyclonal antibody (pAb) (#NB110–89474) and GFAP rabbit pAb (#NB300–141) were procured from Novus Biologicals (Centennial, CO, USA). GAPDH rabbit mAb (#2118S) was purchased from Cell Signaling Technology (Danvers, MA, USA). PathoGreen Histofluorescent stain (#80027–5) was purchased from Biotium (Fremont, CA, USA). Cy3-goat anti-rabbit IgG (#111–165-144) and Cy2-goat anti-mouse IgG (#115–225-146) were procured from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

Richards T., Perron J.C., Patel K., Wurpel J., Reznik S.E, & Schanne F. (2023). Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes. Research Square.

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