Testosterone elisa kit

ESR 100 electrospraying equipment (NanoNC, Seoul, Korea) and gas-tight syringes (Hamilton Co, Reno, NV, USA) were used for the preparation of microspheres. SEM (S-4800; Hitachi Ltd., Tokyo, Japan), differential scanning calorimetry (DSC) (DSC Q20; TA Instruments, New Castle, DE, USA), FTIR spectrophotometer-430 (Shimadzu Corporation, Tokyo, Japan), sputter coater (K575K; EMI Teck, UK), and heating dry bath (confido–S20H, Seoul, Korea) were used for the physicochemical evaluation. The HPLC system for release test and drug content assay consisted of an Alliance^ⓇHPLC e2695 system with 2489 UV/Vis Detector. The UPLC-MS/MS system for bioanalysis consisted of Acquity^™UPLC system (Waters Corp., Milford, MA, USA) coupled with a Mass Spectrometer (Xevo™ TQ-S, Waters Corp., Milford, MA, USA). The testosterone ELISA kit (R&D Systems, Minneapolis, MN, USA) was used for determination of testosterone in rat plasma.

The levels of specific markers of osteogenesis in the serum from WT and Prdx5^Ko mice at 12 weeks were measured using enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s description. The RANKL levels were measured using a mouse RANKL ELISA kit (Abcam); the OPG levels were measured using a Quantikine ELISA (R&D Systems) kit; and the BMP2 levels were measured using mouse BMP2 ELISA kits, respectively (LSBio).
The testosterone levels in the serum from WT and Prdx5^Ko male mice at 4, 8, and 12 weeks were measured using the testosterone ELISA kit (R&D Systems) according to the manufacturer’s description.