A Xevo TQ-S tandem-quadrupole mass spectrometry (Waters, Manchester, United Kingdom) equipped with a Z-spray electrospray interface was used. Positive electrospray ionization was performed in the multiple reaction monitoring (MRM) mode. The capillary voltage was set to 1.0 kV, the source block temperature was set to 120°C, the desolvation gas (nitrogen) was heated to 650°C and delivered at a flow rate of 1000 L/h, and the cone gas (nitrogen) was set to 150 L/h. Dwell times were automatically adjusted with 15–20 data points per peak. MRM transitions, cone voltage, and collision energy for the analytes and IS used in the presented method are shown in Table 1 . System operation and data acquisition were controlled using Mass Lynx 4.1 software (Waters). All data were processed with the Target Lynx quantification program (Waters).
Xevo tq s tandem quadrupole mass spectrometry
Manufactured by Waters Corporation
Sourced in United Kingdom
The Xevo TQ-S is a tandem-quadrupole mass spectrometer manufactured by Waters Corporation. It is designed to perform high-sensitivity, high-resolution mass analysis and quantification of chemical compounds. The core function of the Xevo TQ-S is to ionize, separate, and detect analytes based on their mass-to-charge ratio.
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2 protocols using xevo tq s tandem quadrupole mass spectrometry
1
Tandem-Quadrupole Mass Spectrometry Protocol
2
Quantification of Pregabalin Levels
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Rats (n = 3 per group) were intraperitoneally treated either with 3 or 100 mg/kg of pregabalin, or orally either with 1 or 10 mg/kg of EMA401, or 80 mg/kg of minocycline. At 0.5, 1, and 2 hours after treatment, approximately 150 µL of blood was collected from the jugular vein using a heparinized syringe with a needle. Blood samples were then centrifuged at 1700 g at 4 °C for 10 minutes. Plasma samples were then separated and stored at −20 °C until analysis. Five or 10 μL of plasma samples were diluted with 300 μL of deproteinization solution containing internal standards (5 nM buspirone, 20 nM tolbutamide, 10 nM reserpine in acetonitorile/methanol = 9: 1) and shaken for 5 minutes at room temperature, and centrifuged at 1000 g for 15 minutes. Then, the supernatant was analyzed by LC-MS/MS (Xevo TQ-S Tandem Quadrupole Mass Spectrometry, Waters) for quantification of pregabalin. Data analysis were performed with MassLynx Mass Spectometry Software and TargetLynx Application manager (Waters).
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