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Cd8 sk1 or rpa t8

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CD8 (SK1 or RPA-T8) is a laboratory reagent used to detect and identify CD8+ T cells. It is a monoclonal antibody that specifically binds to the CD8 surface antigen, which is expressed on the surface of cytotoxic T cells and a subset of natural killer cells. This reagent can be used in various immunological assays, such as flow cytometry, to quantify and characterize CD8+ T cell populations.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Live dead fixable dead cell stain, by Thermo Fisher Scientific (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Facsymphony, by BD (2 mentions) Cd45ra hi100, by BioLegend (2 mentions) Granzyme b gb11, by BioLegend (2 mentions) Pd 1 eh12.1, by BioLegend (2 mentions) Ccr7 g043h7, by BioLegend (2 mentions)

Close spelling variants of this product

RPA-T8 (46 citations) CD8 (SK1, (7 citations)

2 protocols using cd8 sk1 or rpa t8

1

Multiparameter Flow Cytometry Analysis

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Single-cell suspensions were washed with PBS and stained with LIVE/DEAD Fixable Dead Cell Stain (Thermo Fisher) for 15 minutes, followed by staining with an optimized antibody cocktail for 20 minutes at room temperature (RT). Cells were then washed with FACS buffer (PBS containing 2% FBS) and fixed in PBS containing 1% paraformaldehyde (Sigma-Aldrich). For samples requiring intracellular staining, cells were processed using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher) according to the manufacture’s instruction. Samples were acquired using a FACSymphony (BD Biosciences). Data were analyzed with FlowJo software (version 9.8.8 or higher, BD Biosciences). All sorting experiments were carried out on freshly isolated cells using a FACSAriaII (BD Biosciences). Antibodies used include: CD45 (HI30), CD103 (Ber-ACT8), CD45RA (HI100, Biolegend), CD3 (UCHT1), CD69 (FN50), CD8 (SK1 or RPA-T8), CCR7 (G043H7, Biolegend), CD25 (M-A251), CD127 (HIL-7R-M21), CD4 (RPA-T4), CCR5 (2D7), PD-1 (EH12.1, Biolegend), Granzyme B (GB11), Foxp3 (259D/C7), CTLA4 (BNI3), IL-17A (N49-653), IL-2 (5344.111), (TNFα (Mab11), IFNγ (B27). All antibodies were from BD Biosciences unless otherwise noted.
Davis A.S., Vick S.C., Pattacini L., Voillet V., Hughes S.M., Lentz G.M., Kirby A.C., Fialkow M.F., Gottardo R., Hladik F., Lund J.M, & Prlic M. (2021). The human memory T cell compartment changes across tissues of the female reproductive tract. Mucosal immunology, 14(4), 862-872.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were washed with PBS and stained with LIVE/DEAD Fixable Dead Cell Stain (Thermo Fisher) for 15 minutes, followed by staining with an optimized antibody cocktail for 20 minutes at room temperature (RT). Cells were then washed with FACS buffer (PBS containing 2% FBS) and fixed in PBS containing 1% paraformaldehyde (Sigma-Aldrich). For samples requiring intracellular staining, cells were processed using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher) according to the manufacture’s instruction. Samples were acquired using a FACSymphony (BD Biosciences). Data were analyzed with FlowJo software (version 9.8.8 or higher, BD Biosciences). All sorting experiments were carried out on freshly isolated cells using a FACSAriaII (BD Biosciences). Antibodies used include: CD45 (HI30), CD103 (Ber-ACT8), CD45RA (HI100, Biolegend), CD3 (UCHT1), CD69 (FN50), CD8 (SK1 or RPA-T8), CCR7 (G043H7, Biolegend), CD25 (M-A251), CD127 (HIL-7R-M21), CD4 (RPA-T4), CCR5 (2D7), PD-1 (EH12.1, Biolegend), Granzyme B (GB11), Foxp3 (259D/C7), CTLA4 (BNI3), IL-17A (N49-653), IL-2 (5344.111), (TNFα (Mab11), IFNγ (B27). All antibodies were from BD Biosciences unless otherwise noted.
Davis A.S., Vick S.C., Pattacini L., Voillet V., Hughes S.M., Lentz G.M., Kirby A.C., Fialkow M.F., Gottardo R., Hladik F., Lund J.M, & Prlic M. (2021). The human memory T cell compartment changes across tissues of the female reproductive tract. Mucosal immunology, 14(4), 862-872.
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