Recombinant mouse TNF-α was acquired from Sigma-Aldrich (654245, St. Louis, MO, USA), while antibodies against Angptl4 were purchased from Sigma-Aldrich (AB10605, St Louis, MO, USA). Antibodies against P65(80979-1-RR), MMP3(18165-1-AP), BAX (50599-2-Ig), Bcl-2(26593-1-AP), cleaved caspase3(19677-1-AP), and Cytochrome c(10993-1-AP) were obtained from Proteintech (Rosemont, IL, USA), while antibodies against C-caspase 3(9661S), P-P65(3039S), and IκBα(9242S) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against GAPDH (ab8245), IL-6 (ab290735), ADAMTS5 (ab41037), Collagen II (ab34712), P-IκBα(ab133462), MMP13 (ab39012), SIRTUIN 1 (ab110304), and Lamin B (ab232731) were obtained from Abcam (Cambridge, MA, USA).
Cytochrome c 10993 1 ap
Manufactured by Proteintech
Sourced in United States
Cytochrome c(10993-1-AP) is a primary antibody that specifically recognizes cytochrome c, a heme-containing protein involved in the electron transport chain of mitochondria. It is commonly used in research applications to detect and study the expression and localization of cytochrome c in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab39012, by Abcam (1 mentions)
Ab41037, by Abcam (1 mentions)
Ab290735, by Abcam (1 mentions)
Ab110304, by Abcam (1 mentions)
Ab34712, by Abcam (1 mentions)
Ab232731, by Abcam (1 mentions)
Recombinant mouse tnf α, by Merck Group (1 mentions)
Ab8245, by Abcam (1 mentions)
Ab133462, by Abcam (1 mentions)
Bcl 2 12789 1 ap, by Proteintech (1 mentions)
Odyssey clx, by Gene Tech (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
β actin, by Proteintech (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
2 protocols using cytochrome c 10993 1 ap
1
Cytokine Signaling Pathway Analysis
2
Western Blot Analysis of Apoptotic Proteins
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For western blot analysis, total protein samples were extracted from heart tissue or cardiomyocytes using radio-immunoprecipitation assay (RIPA) lysis buffer (Beyotime, Shanghai, China) with complete protease inhibitor cocktail (Roche Molecular Biochemicals, Basel, Switzerland). A total of 40–60 μg proteins were fractionated on a 12% SDS-PAGE. After electrophoretically transferring to a Pure Nitrocellulose Blotting membrane (Pall Life Science), the blots were probed with primary antibodies, with β-actin (Proteintech, Wuhan, China) as a loading control. Primary antibodies included Bak1 (14673-1-AP; Proteintech), Bax (5099-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), and cytochrome c (10993-1-AP; Proteintech). The immunoreactivity was detected and analyzed using Odyssey Clx (Gene Company Limited, Hong Kong, China).
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