Sc 32317

Manufactured by Santa Cruz Biotechnology

Sc-32317 is a laboratory reagent produced by Santa Cruz Biotechnology. It is a chemical compound used in scientific research and experimentation. The core function of Sc-32317 is to serve as a tool for researchers in their investigations, but no further details about its intended use or applications can be provided in an unbiased and factual manner.

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Lab products found in correlation

Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Biotinylated rna oligonucleotides, by Biosearch Technologies (1 mentions) Goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Goat anti mouse, by Thermo Fisher Scientific (1 mentions) A16066, by Thermo Fisher Scientific (1 mentions) Dynabeads m 280 streptavidin magnetic beads, by Thermo Fisher Scientific (1 mentions) Enhanced chemiluminescence, by Vazyme (1 mentions) Protease inhibitor cocktail, by Beyotime (1 mentions) Ripa protein lysis buffer, by Beyotime (1 mentions)

2 protocols using sc 32317

Affinity Purification of RNA-Binding Proteins

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Affinity purification of RNA-binding proteins was performed as previously described (17 (link)) using biotinylated RNA oligonucleotides (LGC Biosearch Technologies, Risskov, Denmark, sequences in figure 1 and figure 5)) immobilized on Dynabeads M-280 streptavidin magnetic beads (Invitrogen) and incubated with HeLa cell nuclear extract. The proteins were separated on a pre-cast 4–12% NuPAGE Bis-Tris gel (Invitrogen) and transferred to an Immobilon polyvinylidene fluoride (PDVF) membrane (Millipore). The membrane was then blotted with antibodies against SRSF1 (Zymed/Invitrogen, 535814A) and hnRNPA1 (Sigma-Aldrich, 080M4857) with hnRNPL (Santa Cruz, sc-32317) as a binding control. Secondary antibodies were either goat anti-mouse (ThermoFischer Scientific, A16066) or goat anti-rabbit (ThermoFischer Scientific, A16104).

Holm L.L., Doktor T.K., Flugt K.K., Petersen U.S., Petersen R, & Andresen B.S. (2024). All exons are not created equal—exon vulnerability determines the effect of exonic mutations on splicing. Nucleic Acids Research, 52(8), 4588-4603.

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Western Blotting and ELISA Protocols

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For western blotting assays, the protein of cells and tissues was extracted by radio immunoprecipitation assay (RIPA) protein lysis buffer (Beyotime, Shanghai, China) with freshly added protease inhibitor cocktail and PMSF. After boiling, the supernatants were subjected to SDS-PAGE and transferred to nitrocellulose membranes. After blocking with 5% non-fat milk, membranes were successively incubated with primary and HRP-conjugated secondary antibodies before visualizing bands using enhanced chemiluminescence (Vazyme, Nanjing, China). Antibody for HNRNPL was purchased from Santa Cruze (sc-32317). Antibodies for ATF3 (A13470), KLF10 (A2433) and Flag (AE005) were purchased from Abclonal (China). GAPDH (60004-1-Ig), HRP-conjugated secondary goat anti-mouse (SA00001-1) or goat anti-rabbit (SA00001-2) antibodies were purchased from Proteintech (China).
Enzyme linked immunosorbent assay (ELISA) Cell culture supernatants were collected and analyzed for cytokines production using ELISAs according to the manufacturer's instructions. ELISA kit for human CCL5 was from R&D Systems (USA).

Sun J., Zhang H., Wei W., Xiao X., Huang C., Wang L., Zhong H., Jiang Y., Zheng F., Yang H., Jiang G, & Zhang X. (2023). Regulation of CD8⁺ T cells infiltration and immunotherapy by circMGA/HNRNPL complex in bladder cancer. Oncogene, 42(15).

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