Mb21d1

Manufactured by Qiagen

The Mb21d1 is a laboratory equipment product manufactured by Qiagen. It is designed to perform a specific core function, but a detailed description while maintaining an unbiased and factual approach is not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

Gotaq qpcr master mix, by Promega (3 mentions) Fast sybr green master mix, by Promega (2 mentions) Tri reagent, by Merck Group (2 mentions) Ariamx, by Agilent Technologies (2 mentions) Nlrp3, by Qiagen (1 mentions) Cxcl10, by Qiagen (1 mentions) Ifna2, by Qiagen (1 mentions) Ifnβ1, by Qiagen (1 mentions) Ifna4, by Qiagen (1 mentions) Goscript reverse transcription kit, by Promega (1 mentions) Gapdh, by Qiagen (1 mentions) Ifnα4, by Qiagen (1 mentions)

3 protocols using mb21d1

mRNA Expression Analysis of Innate Immune Genes

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Total mRNA was extracted using TRIzol (TRI-Reagent, Sigma-Aldrich, Germany) and reverse transcribed in cDNA with GoScript™ Reverse Transcription kit (Promega, Wisconsin). Genes mRNA expression were analyzed using GoTaq^®qPCR Master Mix (Promega). All primer sequences used were from Qiagen: Tmem173 (#QT00261590), Mb21d1 (#QT00131929), Nlrp3 (#QT00122458), Ifi204 (#QT01753535), Ddx41 (#QT00137130), Aim2 (#QT00266819), Cxcl10 (#QT00093436), Ifna2 (#QT00253092), Ifna4 (#QT01774353), and Ifnβ1 (#QT00249662). RNA expression was normalized to Rn18s expression (Qiagen, Maryland). Data were analyzed using the comparative analysis of relative expression by ^ΔΔCt methods.

Messaoud-Nacer Y., Culerier E., Rose S., Maillet I., Rouxel N., Briault S., Ryffel B., Quesniaux V.F, & Togbe D. (2022). STING agonist diABZI induces PANoptosis and DNA mediated acute respiratory distress syndrome (ARDS). Cell Death & Disease, 13(3), 269.

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qRT-PCR of STING, cGAS, and Fibronectin

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RNA was purified from lung homogenates using Tri-Reagent (Sigma-Aldrich, Saint-Louis, MO) extraction protocol. RNA reverse transcription into cDNA was carried out with GoTaq qPCR-Master Mix (Promega, Madison, WI). RT-qPCR was performed with Fast SYBR Green Master mix (Promega) on an ARIA MX (Agilent Technologies, Santa Clara, CA). Primers Tmem173 (#QT00261590) encoding for STING, Mb21d1 (#QT00131929) encoding for cGAS, and Fn1 (#QT00135758) encoding for fibronectin were purchased from Qiagen (Qiagen, Hilden, Germany). RNA expression was normalized to Gapdh (#QT00166768, Qiagen, Hilden, Germany) expression and analyzed using the ΔΔCt method.

Savigny F., Schricke C., Lacerda-Queiroz N., Meda M., Nascimento M., Huot-Marchand S., Da Gama Monteiro F., Ryffel B., Gombault A., Le Bert M., Couillin I, & Riteau N. (2021). Protective Role of the Nucleic Acid Sensor STING in Pulmonary Fibrosis. Frontiers in Immunology, 11, 588799.

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Lung RNA Expression Analysis

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RNA was purified from lung homogenates by using Tri-Reagent (Sigma-Aldrich, Saint-Louis, MO) extraction protocol. Reverse transcription of RNA into cDNA was carried out with GoTaq qPCR-Master Mix (Promega, Madison, WI). RT-qPCR was performed with Fast SYBR Green Master mix (Promega) on an ARIA MX (Agilent Technologies, Santa Clara, CA). Primers for Tmem173 (#QT00261590), Mb21d1 (#QT00131929) and Ifnα4 (#QT01774353) were purchased from Qiagen (Qiagen, Hilden, Germany). RNA expression was normalized to Gapdh (#QT00166768) expression and analysed using the ^ΔΔCt method.

Nascimento M., Gombault A., Lacerda-Queiroz N., Panek C., Savigny F., Sbeity M., Bourinet M., Le Bert M., Riteau N., Ryffel B., Quesniaux V.F, & Couillin I. (2019). Self-DNA release and STING-dependent sensing drives inflammation to cigarette smoke in mice. Scientific Reports, 9, 14848.

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