Immunohistochemistry was performed in formalin-fixed, paraffin-embedded, 4-μm-thick heart sections [5 (link), 21 (link), 37 (link)]. Cell proliferation was assessed by immunofluorescent staining of nuclei with specific antibodies for BrdU (Roche). Myocytes were stained with an anti-α-sarcomeric actin (α-SA) antibody (Sigma). Myocyte membranes were stained with Rhodamine-labeled wheat germ agglutinin (WGA) (Vector Labs) to facilitate the identification of individual myocytes for analysis of myocyte cross-sectional area and myocyte density. To determine vessel density, heart sections were stained with Fluorescein-labeled Griffonia simplicifolia Lectin I (GSL I) isolectin B4 (Vector Labs). Double immunofluorescent staining was conducted with specific anti-BrdU and anti- α-SA antibodies for evaluation of proliferating myocytes. Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole).
Anti α sarcomeric actin α sa antibody
Manufactured by Merck Group
The Anti-α-sarcomeric actin (α-SA) antibody is a laboratory reagent used to detect and identify α-sarcomeric actin, a structural protein found in muscle cells. This antibody can be utilized in various immunochemical techniques, such as Western blotting and immunohistochemistry, to study the expression and localization of α-sarcomeric actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine labeled wheat germ agglutinin, by Vector Laboratories (1 mentions)
Alexa fluor 647 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Biotin conjugated goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Anti gfp antibody, by Thermo Fisher Scientific (1 mentions)
Aec substrate kit, by BD (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti α sarcomeric actin α sa antibody
1
Immunohistochemistry of Cardiac Tissues
2
Tracking BM-Derived Cell Transdifferentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To investigate if BM-derived cells can acquire CSCs properties, GFP+ BM-derived cells and CSCs differentiation in BMT heart were tracked by immunostaing. Anti-GFP antibody (Invitrogen), anti-α-sarcomeric actin (α-SA) antibody (Sigma), anti-Sca-1 antibody (Abcam, Cambridge, MA), and anti-c-Kit antibody (Millipore, Billerica, MA) were used. Alexa Fluor 488, Alexa Fluor 594 and Alexa Fluor 647-conjugated secondary antibodies (all from Invitrogen) were applied appropriately. DAPI was used for nuclear counterstaining. To track transdifferentation of BM-derived cells, immunohistochemical staining of GFP expression was carried out. Briefly, the Anti-GFP antibody was labeled with biotin-conjugated goat anti-rabbit IgG (Santa Cruz Biotech, Santa Cruz, CA) and the GFP expressions were detected by the color reaction with AEC Substrate Kit (BD Pharmingen).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!