Log In Sign up
The largest database of trusted experimental protocols

Troponint gcamp5 zeo

Manufactured by Addgene
Visit Supplier

TroponinT-GCaMP5-Zeo is a lab equipment product that serves as a calcium indicator. It is a fusion protein that combines the calcium-binding protein troponin T with the fluorescent protein GCaMP5 and a zeomycin resistance gene.

Automatically generated - may contain errors

Lab products found in correlation

Fudeltagw rtta, by Addgene (1 mentions) Midiprep kit, by Qiagen (1 mentions) Teto gata4, by Addgene (1 mentions) Pgk h2bmcherry, by Addgene (1 mentions) Stbl3 bacteria, by Thermo Fisher Scientific (1 mentions) Pspax2, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions)

2 protocols using troponint gcamp5 zeo

1

AAVS1 Locus Targeting Donor Plasmids Construction

Check if the same lab product or an alternative is used in the 5 most similar protocols
The donor plasmids targeting AAVS1 locus were constructed as previously described (Bao et al., 2019 (link)). Briefly, to generate the CAG-FUCCI plasmid, the Clover-Geminin (1–110)-IRES-mKO2-Cdt (30–120) fragment was amplified from Addgene plasmid #83841 and then cloned into the AAVS1-Pur-CAG-EGFP donor plasmid (Addgene; #80945), replacing the EGFP. For TNNT2-FUCCI plasmid, the cTnT promoter was polymerase chain reaction (PCR) amplified from TroponinT-GCaMP5-Zeo (Addgene; #46027), and then cloned into the CAG-FUCCI plasmid via Gibson Assembly (NEB; #2611S), replacing the CAG promoter. Both FUCCI plasmids were sequenced and submitted to Addgene (#136934 and #136935).
Chang Y., Hellwarth P.B., Randolph L.N., Sun Y., Xing Y., Zhu W., Lian X.L, & Bao X. (2020). Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells. Biotechnology and bioengineering, 117(7), 2177-2186.
+ Open protocol
+ Expand
2

Engineered Cardiac Transcription Factors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All plasmids were constructed as previously described [5] (link) and can be found on Addgene using the following catalog numbers: Troponin T-GCaMP5-Zeo (46027), tetO-Hand2 (46028), tetO-NKX2.5 (46029), tetO-GATA4 (46030), tetO-MEF2C (46031), tetO-TBX5 (46032). Plasmids that were used from Addgene also include: FUdeltaGW-rtTA (19780), psPAX2 (12260), pMD2.G (12259), and PGK-H2B-mCherry (21217). All plasmids were amplified in STBL3 bacteria (Invitrogen) and prepared with Qiagen MidiPrep Kits.
Ifkovits J.L., Addis R.C., Epstein J.A, & Gearhart J.D. (2014). Inhibition of TGFβ Signaling Increases Direct Conversion of Fibroblasts to Induced Cardiomyocytes. PLoS ONE, 9(2), e89678.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!