Blood samples were centrifuged at 3000 rpm/min for 15 min to collect the serum. Then, serum levels of IL-1β, IL-18 and Hcy were determined using an interleukin 1β (IL-1β) assay kit (H002, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), an interleukin 18 (IL-18) assay kit (H015, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), a homocysteine (Hcy) assay kit (E031-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s instructions. In these assays, the standard substance was dissolved in deionized water as a standard stock solution, which was subsequently diluted to 7 different gradient concentrations. The different concentrations of standards and samples were then added to the antibody-covered microplates, and after incubating for 2 h, the microplates were washed 5 times. The chromogenic substrate was then added to the wells, and after incubating for 30 min in the dark, stop solution was added to terminate the reaction. The absorbance values were then detected using a microplate reader (XSZ-02, BioTek, America). IL-1β, IL-18, and Hcy levels were calculated based on their corresponding standard curves.
Il 1β assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The IL-1β assay kit is a laboratory equipment product designed to measure the concentration of the interleukin-1 beta (IL-1β) cytokine in various sample types. It utilizes an enzyme-linked immunosorbent assay (ELISA) technique to quantify the levels of IL-1β in the tested samples.
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Lab products found in correlation
2 protocols using il 1β assay kit
1
Serum Biomarker Quantification Protocol
2
Neurochemical Analysis of Mouse Brain
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Following the behavioral tests, the mice were euthanized, and their brains were isolated and placed in centrifuge tubes immersed on ice, followed by the addition of nine times the volume of normal saline solution. After homogenization with a tissue precision homogenizer, the supernatant was obtained through centrifugation (4 °C, 4000 rpm, 10 min). The contents of GABA, 5-HT, NE, Glutamine (Glu), GAD67, GABAAR, SOD, IL-1β, iNOS in the mouse brain homogenate supernatant were examined by assay kit according to the instructions of the manuscripts. The mouse GABA ELISA Kit (Product code: JL12094), GABAAR ELISA Kit (Product code: JL50569), and GAD67 ELISA Kit (Product code: JL46227) were purchased from Shanghai Jianglaibio Science and Technology Ltd., China; The 5-HT assay Kit (Product code: H104), NE assay Kit (Product code: H096), NOS typed assay kit (Product code: A014-1-2), Glu measurement kit (Product code: A074-1-1), IL-1β Assay Kit (Product code: H002), SOD assay kit (Product code: A001-3-2) and Total protein quantitative assay kit (Product code: A045-2-2) were purchased from Nanjing Jiancheng Bioengineering Institute, China.
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