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Axiozoom confocal microscope

Manufactured by Zeiss

The Axiozoom confocal microscope is a high-performance imaging system designed for advanced research applications. It utilizes confocal technology to provide optical sectioning and high-resolution imaging capabilities. The core function of the Axiozoom is to enable detailed visualization and analysis of various biological and materials science samples.

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2 protocols using axiozoom confocal microscope

1

Bioprinted Cartilage Tissue Characterization

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The bioprinted constructs were visualized by florescent imaging of GFP+ MSCs using the Zeiss Axiozoom confocal microscope. H&E and immunohistochemistry staining were carried out to visualize the morphology as described in sections “Histological analysis of spheroids” and “Immunohistochemistry of the bioprinted cartilage tissues,” respectively. For the immunohistochemistry staining, anti-Sp7/OSTERIX primary antibody (1:500 in 2.5% NGS) and goat anti-rabbit Alexa Fluor 568 secondary antibody (1:200 in 2.5% NGS) were used. Samples were imaged using the Zeiss LSM 880 Airyscan Confocal microscope (Zeiss, Oberkochen, Germany). RT-qPCR of bioprinted tissues were conducted for OSTERIX, COL-1, BSP, and BMP-4 genes as described in section “Gene expression of osteogenic spheroids using real time polymerase chain reaction (RT-qPCR).”
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2

Bioprinted Cartilage Tissue Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The bioprinted constructs were visualized by florescent imaging of GFP+ MSCs using the Zeiss Axiozoom confocal microscope. H&E and immunohistochemistry staining were carried out to visualize the morphology as described in sections “Histological analysis of spheroids” and “Immunohistochemistry of the bioprinted cartilage tissues,” respectively. For the immunohistochemistry staining, anti-Sp7/OSTERIX primary antibody (1:500 in 2.5% NGS) and goat anti-rabbit Alexa Fluor 568 secondary antibody (1:200 in 2.5% NGS) were used. Samples were imaged using the Zeiss LSM 880 Airyscan Confocal microscope (Zeiss, Oberkochen, Germany). RT-qPCR of bioprinted tissues were conducted for OSTERIX, COL-1, BSP, and BMP-4 genes as described in section “Gene expression of osteogenic spheroids using real time polymerase chain reaction (RT-qPCR).”
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