The excision specimen from the left foot comprising skin and subcutaneous tissue was received in buffered neutral formalin. The tumor in the specimen was extensively sampled and processed for routine hematoxylin and eosin–stained sections. Five-μm sections from formalin-fixed, paraffin-embedded tissues were stained with antibodies against CD31 (Neomarker), CD34 (DAKO), ERG (Cell Marque), STAT6 (Cell Marque), Factor XIIIa (Cell Marque), CD163 (Cell Marque), desmin (DAKO), and SMA (DAKO) using the Ventana Optiview detection kit on the Roche Ventana BenchMark ULTRA slide stainer (Roche Diagnostics) after antigen retrieval, if required. For CD34, STAT6, Factor XIIIa, CD163, and desmin stains, the ULTRA cell conditioning method on the Roche Ventana BenchMark ULTRA slide stainer was used for antigen retrieval. For ERG and CD31 stains, the pressure cooker with citrate buffer at pH6 method was employed. For SMA, no antigen retrieval treatment was required.
Stat6
Manufactured by Roche
Sourced in United States
STAT6 is a product that measures the levels of the STAT6 protein in biological samples. STAT6 is a transcription factor that plays a role in immune system regulation. The STAT6 product provides quantitative data on STAT6 expression, which can be used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Desmin, by Agilent Technologies (1 mentions)
Desmin, by Roche (1 mentions)
Cd163, by Cell Marque (1 mentions)
Factor xiiia, by Cell Marque (1 mentions)
Ventana benchmark ultra slide stainer, by Roche (1 mentions)
Cd163, by Roche (1 mentions)
Mouse anti il13rα2, by Santa Cruz Biotechnology (1 mentions)
Mouse anti ha, by Roche (1 mentions)
Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Mouse anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Goat anti il13rα2, by R&D Systems (1 mentions)
Pcmv6 empty vector, by OriGene (1 mentions)
2 protocols using stat6
1
Immunohistochemical Analysis of Skin Tumor
2
Antibody Sourcing for IL13Rα2 Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies were purchased from the following sources: goat anti-IL13Rα2 (R&D Systems, Minneapolis, MN, USA), mouse anti-IL13Rα2, mouse anti-α-tubulin, and mouse anti-GAPDH (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), STAT6, P-STAT1, P-STAT3, P-STAT5, P-STAT6 (S737), P-STAT6 (Y641), mouse anti-HA (Roche, Basel, Switzerland), and anti-TP63α (Cell Signaling Technology, Beverly, MA, USA). PCMV6 empty vector or pCMV6-IL13Rα2-Myc-Flag constructs were purchased from OriGene (Rockville, MD, USA).
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