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Wst assays

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WST assays are a colorimetric method for the quantitative determination of cell viability and proliferation. The assay utilizes the tetrazolium compound WST-1, which is cleaved to a colored formazan dye by mitochondrial dehydrogenases in viable cells. The amount of formazan dye formed is directly proportional to the number of metabolically active cells in the sample.

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Lab products found in correlation

Ipc298, by Leibniz Institute DSMZ (1 mentions) Alamar blue, by Thermo Fisher Scientific (1 mentions) Incucyte zoom system, by Sartorius (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Igr39, by Leibniz Institute DSMZ (1 mentions) Fetal calf serum (fcs), by GE Healthcare (1 mentions) Du145, by Leibniz Institute DSMZ (1 mentions) Hek293, by Leibniz Institute DSMZ (1 mentions) Sh sy5y, by Leibniz Institute DSMZ (1 mentions) Imr 32, by Leibniz Institute DSMZ (1 mentions) Power sybr green master mix, by Thermo Fisher Scientific (1 mentions) 7900ht real time pcr system, by Thermo Fisher Scientific (1 mentions) Lncap, by American Type Culture Collection (1 mentions) Du145, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

WST-1-based colorimetric assay WST-1 colorimetric assay for cell proliferation Premix WST-1 Cell Proliferation Assay System WST-1 cytotoxicity assay WST-1 cell viability assay WST-1 viability assay WST-1 cell viability assay kit WST-1 colorimetric assay Colorimetric WST-1 cell proliferation assay WST-1 assay

2 protocols using wst assays

1

Cell Line Characterization and Proliferation Assays

Cited 2 times
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Cell lines DU145 (ACC 261), HEK293 (ACC 305), HeLa (ACC 57), IPC298 (ACC 251), IGR39 (ACC 239), IMR32 (ACC 165), and SH-SY5Y (ACC 209) were purchased from DSMZ (Braunschweig, Germany). MDA-MB-435S (HTB129) cells were from ATCC (Manassas, VA), PNT2 cells (ECACC95012613) were obtained from ECACC (Salisbury, UK). GL15 cells were kindly provided by Dr. Fioretti (University of Perugia, Italy). Each cell line was cultured in their respective recommended medium supplemented with 10% FCS (PAA Laboratories) at 37 °C in humidified 5% CO2 atmosphere. For stablly transfected cell lines (HEK expressing KV10.1 in the pTracerCMV vector, a cell line routinely used in our laboratory (4 (link), 6 (link)– (link)8 (link)), the selection compound Zeocin (Calya) was added to the culture medium at 3 μg/ml. Transient transfections were performed using FuGENE (Roche Applied Science) or Lipofectamine 2000 (Invitrogen). Proliferation was estimated using Alamar Blue (BIOSOURCE) or WST assays (Roche Applied Science) as described (50 (link)) or by live cell imaging in an IncuCyte Zoom system (Essen Biosciences) to determine the percent confluence as a function of time.
Ramos Gomes F., Romaniello V., Sánchez A., Weber C., Narayanan P., Psol M, & Pardo L.A. (2015). Alternatively Spliced Isoforms of KV10.1 Potassium Channels Modulate Channel Properties and Can Activate Cyclin-dependent Kinase in Xenopus Oocytes. The Journal of Biological Chemistry, 290(51), 30351-30365.
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2

Prostate Cancer Cell Line Chemosensitivity Assay

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LNCaP, Du145, PC3, and RWPE cell lines were obtained from the American Type Culture Collection (ATCC) and maintained in standard conditions. Stable overexpression and knockdown cell lines were generated with lentiviral constructs with blasticidin or puromycin selection as appropriate. RNA isolation and cDNA synthesis were performed according to standard protocols. Quantitative PCR was performed with Power SYBR Green Mastermix on an Applied Biosystems 7900HT Real-Time PCR system. Chemosensitivity assays were performed on 5000 cells plated per well in 96 well plates and treated with a single dose of Olaparib or ABT-888 as indicated for 72 hours. WST assays (Roche) were performed according to the manufacturer’s instructions. Immunofluorescence experiments were performed with 1 x 105 cells in 12-well plates according to standard protocols; RAD51 and γ-H2AX staining was performed 6 hours or 24 hours post-treatment, respectively.
Prensner J.R., Chen W., Iyer M.K., Cao Q., Ma T., Han S., Sahu A., Malik R., Wilder-Romans K., Navone N., Logothetis C.J., Araujo J.C., Pisters L.L., Tewari A.K., Canman C.E., Knudsen K.E., Kitabayashi N., Rubin M.A., Demichelis F., Lawrence T.S., Chinnaiyan A.M, & Feng F.Y. (2014). PCAT-1, a long noncoding RNA, regulates BRCA2 and controls homologous recombination in cancer. Cancer research, 74(6), 1651-1660.
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