Atp colorimetry assay kit

Manufactured by Abcam

Sourced in United States

The ATP Colorimetry Assay Kit is a laboratory tool used to measure adenosine triphosphate (ATP) levels. It provides a quantitative assessment of ATP concentration in samples.

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Lab products found in correlation

Deproteinizing sample preparation kit, by Abcam (3 mentions)

4 protocols using atp colorimetry assay kit

Intracellular ATP Quantification in Colonocytes

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For measuring intracellular ATP levels, primary colonocytes were isolated as described above and deproteinized by using a Deproteinizing Sample Preparation Kit (Biovision, Milpitas, CA, USA). ATP measurements in colonocyte lysates were performed by using ATP Colorimetry Assay Kit (Biovision, Milpitas, CA, USA) according to the manufacturer’s instructions.

Alfonso-Garcia A., Cevallos S.A., Lee J.Y., Li C., Bec J., Bäumler A.J, & Marcu L. (2022). Assessment of Murine Colon Inflammation Using Intraluminal Fluorescence Lifetime Imaging. Molecules, 27(4), 1317.

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Intracellular ATP Quantification in Colonocytes

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For measuring intracellular ATP levels, primary colonocytes were isolated as described above and then deproteinized by using a deproteinizing sample preparation kit (BioVision, Milpitas, CA) according to the manufacturer’s instructions. Lactate measurements in colonocyte lysates were performed by using an ATP colorimetry assay kit (BioVision, Milpitas, CA) according to the manufacturer’s instructions.

Cevallos S.A., Lee J.Y., Velazquez E.M., Foegeding N.J., Shelton C.D., Tiffany C.R., Parry B.H., Stull-Lane A.R., Olsan E.E., Savage H.P., Nguyen H., Ghanaat S.S., Byndloss A.J., Agu I.O., Tsolis R.M., Byndloss M.X, & Bäumler A.J. (2021). 5-Aminosalicylic Acid Ameliorates Colitis and Checks Dysbiotic Escherichia coli Expansion by Activating PPAR-γ Signaling in the Intestinal Epithelium. mBio, 12(1), e03227-20.

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Intracellular ATP Measurement in Colonocytes

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For determination of intracellular ATP measurements, primary colonocytes were isolated as described above. ATP measurement in colonocyte lysates was performed by using a ATP Colorimetry Assay Kit (Biovision), according to manufacturer’s instructions.

Byndloss M.X., Olsan E.E., Rivera-Chávez F., Tiffany C.R., Cevallos S.A., Lokken K.L., Torres T.P., Byndloss A.J., Faber F., Gao Y., Litvak Y., Lopez C.A., Xu G., Napoli E., Giulivi C., Tsolis R.M., Revzin A., Lebrilla C, & Bäumler A.J. (2017). Microbiota-activated PPAR-γ-signaling inhibits dysbiotic Enterobacteriaceae expansion. Science (New York, N.Y.), 357(6351), 570-575.

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Intracellular ATP Quantification in Colonocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

For measuring intracellular ATP levels, primary colonocytes were isolated as described above and then deproteinized by using Deproteinizing Sample Preparation Kit (Biovision, Milpitas, CA) following manufacturer’s instructions. Lactate measurement in colonocyte lysates were performed by using ATP Colorimetry Assay Kit (Biovision, Milpitas, CA) according to the manufacturer’s instructions.

Lee J.Y., Cevallos S.A., Byndloss M.X., Tiffany C.R., Olsan E.E., Butler B.P., Young B.M., Rogers A.W., Nguyen H., Kim K., Choi S.W., Bae E., Lee J.H., Min U.G., Lee D.C, & Bäumler A.J. (2020). High-fat diet and antibiotics cooperatively impair mitochondrial bioenergetics to trigger dysbiosis that exacerbates pre-Inflammatory Bowel Disease. Cell host & microbe, 28(2), 273-284.e6.

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