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2 protocols using n acetyl cysteine (nac)

1

Metal Ion Supplementation Protocol

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Puromycin Dihydrochloride (>99%) was purchased from P212121. Zinc Sulfate was purchased as a 100 mM aq. Soln. from Alfa Aesar. Cobalt(II) sulfate heptahydrate (>99%) was purchased from Sigma-Aldrich: SIAL. Magnesium(II) chloride hexahydrate (ACS grade, >99%) was purchased from Amresco. Ferrous ammonium sulfate (99%) was purchased from Santa Cruz Biotechnology. Sodium chloride (99.5%) was purchased from Fisher Scientific. Nickel(II) sulfate (99.99%), Fe(II) bromide (98%), Fe(III) sulfate hydrate (97%), copper(II) chloride (99.999%), copper(I) chloride (>99.995%), calcium chloride (>97%), potassium chloride (>99%), and manganese(II) chloride (98%) were all purchased from Sigma Aldrich. N-acetyl cysteine (NAC) was purchased from TCI America (>98%). Ascorbic Acid was purchased from Chem Impex Int’l (99.8%). Buthionine sulphoximine (BSO) (>98%) was purchased from Cayman Chemical and β-mercaptoethanol (BME) (>98%) was purchased from BioRad. Hepcidin was purchased from abcam (1 mg/mL, 70–90%).
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2

Metal Ion Supplementation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Puromycin Dihydrochloride (>99%) was purchased from P212121. Zinc Sulfate was purchased as a 100 mM aq. Soln. from Alfa Aesar. Cobalt(II) sulfate heptahydrate (>99%) was purchased from Sigma-Aldrich: SIAL. Magnesium(II) chloride hexahydrate (ACS grade, >99%) was purchased from Amresco. Ferrous ammonium sulfate (99%) was purchased from Santa Cruz Biotechnology. Sodium chloride (99.5%) was purchased from Fisher Scientific. Nickel(II) sulfate (99.99%), Fe(II) bromide (98%), Fe(III) sulfate hydrate (97%), copper(II) chloride (99.999%), copper(I) chloride (>99.995%), calcium chloride (>97%), potassium chloride (>99%), and manganese(II) chloride (98%) were all purchased from Sigma Aldrich. N-acetyl cysteine (NAC) was purchased from TCI America (>98%). Ascorbic Acid was purchased from Chem Impex Int’l (99.8%). Buthionine sulphoximine (BSO) (>98%) was purchased from Cayman Chemical and β-mercaptoethanol (BME) (>98%) was purchased from BioRad. Hepcidin was purchased from abcam (1 mg/mL, 70–90%).
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